Open Access

Identification of differentially expressed microRNAs in knee anterior cruciate ligament tissues surgically removed from patients with osteoarthritis

  • Authors:
    • Bin Li
    • Lunhao Bai
    • Peng Shen
    • Yue Sun
    • Zhizuo Chen
    • Yu Wen
  • View Affiliations

  • Published online on: July 31, 2017     https://doi.org/10.3892/ijmm.2017.3086
  • Pages: 1105-1113
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The degradation of cruciate ligaments is frequently observed in degenerative joint diseases, such as osteo­arthritis (OA). The present study aimed to identify the differentially expressed microRNAs (miRNAs or miRs) in knee anterior cruciate ligament (ACL) tissues derived from patients with OA and in health subjects (non-OA). By using Affymetrix miRNA 4.0 microarrays, a total of 22 miRNAs (including let-7f-5p, miR-26b-5p and miR-146a-5p) were found to be upregulated, while 17 (including miR-18a-3p, miR-138-5p and miR-485-3p) were downregulated in the osteoarthritic ACL tissues (fold change ≥2, P-value <0.05). The expression levels of 12 miRNAs were validated by quantitative PCR, and the corresponding results revealed an excellent correlation with the microarray data (R2=0.889). Genes (such as a disintegrin and metalloproteinase domain with thrombospondin type-1 motifs, bone morphogenetic protein-2, runt related transcription factor-2, collagen-1A1 and 2, interleukin-6 and transforming growth factor-β) involved in cartilage development and remodeling, collagen biosynthesis and degradation, inflammatory response and extracellular matrix homeostasis were predicted as potential targets of the dysregulated miRNAs. Moreover, a large set of putative genes were enriched in OA pathogenesis‑associated pathways (such as mitogen-activated protein kinase and vascular endothelial growth factor signaling pathway). Collectively, the data from our study provides novel insight into the ligament injury-related miRNA dysregulation in patients with OA.
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October-2017
Volume 40 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Li B, Bai L, Shen P, Sun Y, Chen Z and Wen Y: Identification of differentially expressed microRNAs in knee anterior cruciate ligament tissues surgically removed from patients with osteoarthritis. Int J Mol Med 40: 1105-1113, 2017
APA
Li, B., Bai, L., Shen, P., Sun, Y., Chen, Z., & Wen, Y. (2017). Identification of differentially expressed microRNAs in knee anterior cruciate ligament tissues surgically removed from patients with osteoarthritis. International Journal of Molecular Medicine, 40, 1105-1113. https://doi.org/10.3892/ijmm.2017.3086
MLA
Li, B., Bai, L., Shen, P., Sun, Y., Chen, Z., Wen, Y."Identification of differentially expressed microRNAs in knee anterior cruciate ligament tissues surgically removed from patients with osteoarthritis". International Journal of Molecular Medicine 40.4 (2017): 1105-1113.
Chicago
Li, B., Bai, L., Shen, P., Sun, Y., Chen, Z., Wen, Y."Identification of differentially expressed microRNAs in knee anterior cruciate ligament tissues surgically removed from patients with osteoarthritis". International Journal of Molecular Medicine 40, no. 4 (2017): 1105-1113. https://doi.org/10.3892/ijmm.2017.3086