Open Access

Transcription factor TBX18 promotes adult rat bone mesenchymal stem cell differentiation to biological pacemaker cells

  • Authors:
    • Yanjun Li
    • Mei Yang
    • Gege Zhang
    • Le Li
    • Bingjie Ye
    • Congxin Huang
    • Yanhong Tang
  • View Affiliations

  • Published online on: November 16, 2017     https://doi.org/10.3892/ijmm.2017.3259
  • Pages: 845-851
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Bone mesenchymal stem cells (BMSCs) are currently considered the optimal stem cells for biological pacemaker cell transformation. The cardiac‑specific transcription factor T‑Box protein 18 (TBX18) is essential for sinoatrial node (SAN) formation, particularly formation of the head region that generates the electrical impulses that induce heart contraction. The present study aimed to confirm the effects of TBX18 on biological pacemaker differentiation of rat BMSCs. Flow cytometry was used to identify the surface markers of BMSCs, in order to acquire pure mesenchymal stem cells. Subsequently, BMSCs were transduced with TBX18 or green fluorescent protein adenovirus vectors. The effects of TBX18 were evaluated using SAN‑specific makers including TBX18, α‑actin, cardiac troponin I, hyperpolarization‑activated cyclic nucleotide‑gated channel 4 and connexin 43 by reverse transcription‑quantitative polymerase chain reaction, western blotting and immunofluorescence. The findings demonstrated that direct conversion of BMSCs to biological pacemaker cells via TBX18 is a feasible method in the field of cardiology.
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February-2018
Volume 41 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Li Y, Yang M, Zhang G, Li L, Ye B, Huang C and Tang Y: Transcription factor TBX18 promotes adult rat bone mesenchymal stem cell differentiation to biological pacemaker cells. Int J Mol Med 41: 845-851, 2018
APA
Li, Y., Yang, M., Zhang, G., Li, L., Ye, B., Huang, C., & Tang, Y. (2018). Transcription factor TBX18 promotes adult rat bone mesenchymal stem cell differentiation to biological pacemaker cells. International Journal of Molecular Medicine, 41, 845-851. https://doi.org/10.3892/ijmm.2017.3259
MLA
Li, Y., Yang, M., Zhang, G., Li, L., Ye, B., Huang, C., Tang, Y."Transcription factor TBX18 promotes adult rat bone mesenchymal stem cell differentiation to biological pacemaker cells". International Journal of Molecular Medicine 41.2 (2018): 845-851.
Chicago
Li, Y., Yang, M., Zhang, G., Li, L., Ye, B., Huang, C., Tang, Y."Transcription factor TBX18 promotes adult rat bone mesenchymal stem cell differentiation to biological pacemaker cells". International Journal of Molecular Medicine 41, no. 2 (2018): 845-851. https://doi.org/10.3892/ijmm.2017.3259