Changed immune and miRNA response in RAW264.7 cells infected with cell wall deficient mycobacterium tuberculosis

  • Authors:
    • Zhengjun Yi
    • Kunshan Gao
    • Ruifang Li
    • Yurong Fu
  • View Affiliations

  • Published online on: February 7, 2018     https://doi.org/10.3892/ijmm.2018.3471
  • Pages: 2885-2892
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Abstract

Cell wall deficient (CWD) forms of Mycobacterium tuberculosis (Mtb) confers a marked resistance to immune system of the host. However, there is limit data on the effect of intracellular CWD-Mtb infection on macrophages. In the study, effects of CWD-Mtb on cell viability, cytokine response and miRNA expression of macrophages were analyzed. Cell viability was reduced, levels of interleukin-1α (IL-1α), IL-1β, IL-6, IL-10 and interferon-γ (IFN-γ) were also significantly changed after infection of RAW264.7 cells with CWD-Mtb. A total of 105 miRNAs were deregulated between CWD-Mtb and wild Mtb group, and among them, miR-29b was upregulated in CWD-Mtb group. Downregulation of miR-29b resulted in significant elevation level of IFN-γ mRNA. Involved signaling pathways of potential target genes of differentially expressed miRNAs mainly focused on T cell receptor signaling pathway, MAPK signaling pathway, neurotrophin signaling pathway, and regulation of actin cytoskeleton. Taken together, the results showed that cytokine production of CWD-Mtb infected macrophages was altered and many miRNAs were involved in regulation of macrophage response to CWD-Mtb infection, which probably determined the differential outcome following different phenotype Mtb infection. These findings open up a new and interesting avenue for an improved understanding of pathogenesis of CWD-Mtb.
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May-2018
Volume 41 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yi Z, Gao K, Li R and Fu Y: Changed immune and miRNA response in RAW264.7 cells infected with cell wall deficient mycobacterium tuberculosis. Int J Mol Med 41: 2885-2892, 2018
APA
Yi, Z., Gao, K., Li, R., & Fu, Y. (2018). Changed immune and miRNA response in RAW264.7 cells infected with cell wall deficient mycobacterium tuberculosis. International Journal of Molecular Medicine, 41, 2885-2892. https://doi.org/10.3892/ijmm.2018.3471
MLA
Yi, Z., Gao, K., Li, R., Fu, Y."Changed immune and miRNA response in RAW264.7 cells infected with cell wall deficient mycobacterium tuberculosis". International Journal of Molecular Medicine 41.5 (2018): 2885-2892.
Chicago
Yi, Z., Gao, K., Li, R., Fu, Y."Changed immune and miRNA response in RAW264.7 cells infected with cell wall deficient mycobacterium tuberculosis". International Journal of Molecular Medicine 41, no. 5 (2018): 2885-2892. https://doi.org/10.3892/ijmm.2018.3471