Black rice (Oryza sativa L.) extract modulates ultraviolet-induced expression of matrix metalloproteinases and procollagen in a skin cell model

  • Authors:
    • Mira Han
    • Jung‑Soo Bae
    • Jae‑Jun Ban
    • Hee Soon Shin
    • Dong Hun Lee
    • Jin Ho Chung
  • View Affiliations

  • Published online on: February 22, 2018     https://doi.org/10.3892/ijmm.2018.3508
  • Pages: 3073-3080
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Exposure of the skin to ultraviolet (UV) radiation causes extracellular matrix (ECM) collapse in the dermis, owing to an increase in matrix metalloproteinase (MMP) production in both the epidermis and dermis, and a decrease in type I collagen expression in the dermis. Recently, black rice (Oryza sativa L.) was reported to have a wide range of pharmacological effects in various settings. However, the effects of black rice extract (BRE) on UV‑irradiated skin cells have not yet been characterized. BRE treatment did not affect cell morphology and viability of HaCaT and human dermal fibroblasts (HDF). We demonstrated that BRE downregulated basal and UV‑induced MMP‑1 expression in HaCaT cells. Furthermore, BRE significantly increased type I procollagen expression, and decreased MMP‑1 and MMP‑3 expression in UV‑irradiated HDF. The underlying mechanisms of these results involve a decrease in p38 and c‑Jun N‑terminal kinase activity, and suppression of UV‑induced activation of activator protein‑1 (AP‑1). BRE reduced UV‑induced reactive oxygen species production in HaCaT cells in a dose‑dependent manner. Indeed, mass spectrometry revealed that BRE contained antioxidative flavonoid components such as cyanidin‑3‑O‑β‑D‑glycoside and taxifolin‑7‑O‑glucoside. These findings suggest that BRE attenuates UV‑induced ECM damage by modulating mitogen‑activated protein kinase and AP‑1 signaling, and could be used as an active ingredient for preventing photoaging of the skin.
View Figures
View References

Related Articles

Journal Cover

May-2018
Volume 41 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Han M, Bae JS, Ban JJ, Shin HS, Lee DH and Chung JH: Black rice (Oryza sativa L.) extract modulates ultraviolet-induced expression of matrix metalloproteinases and procollagen in a skin cell model. Int J Mol Med 41: 3073-3080, 2018
APA
Han, M., Bae, J., Ban, J., Shin, H.S., Lee, D.H., & Chung, J.H. (2018). Black rice (Oryza sativa L.) extract modulates ultraviolet-induced expression of matrix metalloproteinases and procollagen in a skin cell model. International Journal of Molecular Medicine, 41, 3073-3080. https://doi.org/10.3892/ijmm.2018.3508
MLA
Han, M., Bae, J., Ban, J., Shin, H. S., Lee, D. H., Chung, J. H."Black rice (Oryza sativa L.) extract modulates ultraviolet-induced expression of matrix metalloproteinases and procollagen in a skin cell model". International Journal of Molecular Medicine 41.5 (2018): 3073-3080.
Chicago
Han, M., Bae, J., Ban, J., Shin, H. S., Lee, D. H., Chung, J. H."Black rice (Oryza sativa L.) extract modulates ultraviolet-induced expression of matrix metalloproteinases and procollagen in a skin cell model". International Journal of Molecular Medicine 41, no. 5 (2018): 3073-3080. https://doi.org/10.3892/ijmm.2018.3508