Open Access

In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells

  • Authors:
    • Jing Li
    • Chang‑Kui Sun
  • View Affiliations

  • Published online on: October 10, 2018     https://doi.org/10.3892/ijmm.2018.3925
  • Pages: 3364-3370
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

microRNA (miRNA)‑26a‑loaded liposomes were prepared in the present study for effective treatment of leukemia. The results demonstrated that miRNA‑26a reduced the viability of chronic lymphocytic leukemia (CLL) cells in a concentration‑dependent manner. Cells treated with miRNA‑26a‑loaded liposomes exhibited increased rates of apoptosis, as determined by flow cytometry and Hoechst 33342 staining. Western blot analysis revealed an increased apoptotic effect of miRNA‑26a‑loaded liposomes compared with control. Treatment with these liposomes resulted in significant downregulation of the expression of the miRNA‑26a target genes, myeloid cell leukemia 1 and cyclin‑dependent kinase 6. Taken together, the results of the present study indicate that miRNA‑26a exerts apoptosis‑inducing and anticancer effects on leukemia cells, suggesting therapeutic potential. This approach may be possible to extrapolate to other neoplasms, including lymphomas and acute myeloid leukemia.
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December-2018
Volume 42 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Li J and Li J: In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells. Int J Mol Med 42: 3364-3370, 2018
APA
Li, J., & Li, J. (2018). In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells. International Journal of Molecular Medicine, 42, 3364-3370. https://doi.org/10.3892/ijmm.2018.3925
MLA
Li, J., Sun, C."In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells". International Journal of Molecular Medicine 42.6 (2018): 3364-3370.
Chicago
Li, J., Sun, C."In vitro analysis of microRNA-26a in chronic lymphocytic leukemia cells". International Journal of Molecular Medicine 42, no. 6 (2018): 3364-3370. https://doi.org/10.3892/ijmm.2018.3925