Open Access

Insulin gene enhancer binding protein 1 induces adipose tissue‑derived stem cells to differentiate into pacemaker‑like cells

  • Authors:
    • Jian Zhang
    • Mei Yang
    • An‑Kang Yang
    • Xi Wang
    • Yan‑Hong Tang
    • Qing‑Yan Zhao
    • Teng Wang
    • Yu‑Ting Chen
    • Cong‑Xin Huang
  • View Affiliations

  • Published online on: November 27, 2018     https://doi.org/10.3892/ijmm.2018.4002
  • Pages: 879-889
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hybrid approaches combining gene‑ and cell‑based therapies to make biological pacemakers are a promising therapeutic avenue for bradyarrhythmia. The present study aimed to direct adipose tissue‑derived stem cells (ADSCs) to differentiate specifically into cardiac pacemaker cells by overexpressing a single transcription factor, insulin gene enhancer binding protein 1 (ISL‑1). In the present study, the ADSCs were transfected with ISL‑1 or mCherry fluorescent protein lentiviral vectors and co‑cultured with neonatal rat ventricular cardiomyocytes (NRVMs) in vitro for 5‑7 days. The feasibility of regulating the differentiation of ADSCs into pacemaker‑like cells by overexpressing ISL‑1 was evaluated by observation of cell morphology and beating rate, reverse transcription‑quantitative polymerase chain reaction analysis, western blotting, immunofluorescence and analysis of electrophysiological activity. In conclusion, these data indicated that the overexpression of ISL‑1 in ADSCs may enhance the pacemaker phenotype and automaticity in vitro, features which were significantly increased following co‑culture induction.
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February-2019
Volume 43 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Zhang J, Yang M, Yang AK, Wang X, Tang YH, Zhao QY, Wang T, Chen YT and Huang CX: Insulin gene enhancer binding protein 1 induces adipose tissue‑derived stem cells to differentiate into pacemaker‑like cells. Int J Mol Med 43: 879-889, 2019
APA
Zhang, J., Yang, M., Yang, A., Wang, X., Tang, Y., Zhao, Q. ... Huang, C. (2019). Insulin gene enhancer binding protein 1 induces adipose tissue‑derived stem cells to differentiate into pacemaker‑like cells. International Journal of Molecular Medicine, 43, 879-889. https://doi.org/10.3892/ijmm.2018.4002
MLA
Zhang, J., Yang, M., Yang, A., Wang, X., Tang, Y., Zhao, Q., Wang, T., Chen, Y., Huang, C."Insulin gene enhancer binding protein 1 induces adipose tissue‑derived stem cells to differentiate into pacemaker‑like cells". International Journal of Molecular Medicine 43.2 (2019): 879-889.
Chicago
Zhang, J., Yang, M., Yang, A., Wang, X., Tang, Y., Zhao, Q., Wang, T., Chen, Y., Huang, C."Insulin gene enhancer binding protein 1 induces adipose tissue‑derived stem cells to differentiate into pacemaker‑like cells". International Journal of Molecular Medicine 43, no. 2 (2019): 879-889. https://doi.org/10.3892/ijmm.2018.4002