Open Access

Differentiation of CD45‑/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro

  • Authors:
    • Yang Xu
    • Ping Sun
    • Jian‑Yu Wang
    • Zong‑Ze Li
    • Rui‑Lan Gao
    • Xue‑Zhe Wang
    • William D. Phillips
    • Simon X. Liang
  • View Affiliations

  • Published online on: January 8, 2019     https://doi.org/10.3892/ijmm.2019.4053
  • Pages: 1128-1138
  • Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Side population (SP) cells are a small subpopulation of cells found in many mammalian tissues and organs, identified by their capacity to efflux Hoechst 33342 dye. They are enriched for stem/progenitor cell activity. SP cells isolated from the adult mouse lung can be separated into a CD45+ subset (bone marrow‑derived) and a CD45‑ subset that can be subdivided into CD31‑ and CD31+ subpopulations. CD45‑/CD31‑ lung SP (LSP) cells are known to be mesenchymal stem cells. However, CD45‑/CD31+ LSP cells are not fully characterized. In the present study, it was found that CD45‑/CD31+ LSP cells were able to form colonies. Based on the expression of vascular endothelial growth factor receptor 2 (VEGFR2), these cells were separated into VEGFR2‑ and VEGFR2+ cells. The CD45‑/CD31+/VEGFR2‑ LSP cells expressed genes characteristic of smooth muscle and endothelial progenitors, and were able to differentiate into smooth muscle and endothelial cells in vitro. The CD45‑/CD31+/VEGFR2+ LSP cells expressed genes characteristic of endothelial progenitors and gave rise to endothelial cells, although not smooth muscle, in vitro. The data demonstrate that CD45‑/CD31+/VEGFR2‑ LSP cells differentiated into CD45‑/CD31+/VEGFR2+ LSP cells and then endothelial cells, indicating that CD45‑/CD31+/VEGFR2+ LSP cells are likely to be derived from CD45‑/CD31+/VEGFR2‑ LSP cells. Taken together, the results suggest that CD45‑/CD31+ LSP cells can be separated into CD45‑/CD31+/VEGFR2‑ LSP cells, which may be progenitors of endothelial and smooth muscle, whereas CD45‑/CD31+/VEGFR2+ LSP cells may serve as late commitment endothelial progenitors in the adult mouse lung.
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March-2019
Volume 43 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Copy and paste a formatted citation
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Spandidos Publications style
Xu Y, Sun P, Wang JY, Li ZZ, Gao RL, Wang XZ, Phillips WD and Liang SX: Differentiation of CD45‑/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro. Int J Mol Med 43: 1128-1138, 2019
APA
Xu, Y., Sun, P., Wang, J., Li, Z., Gao, R., Wang, X. ... Liang, S.X. (2019). Differentiation of CD45‑/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro. International Journal of Molecular Medicine, 43, 1128-1138. https://doi.org/10.3892/ijmm.2019.4053
MLA
Xu, Y., Sun, P., Wang, J., Li, Z., Gao, R., Wang, X., Phillips, W. D., Liang, S. X."Differentiation of CD45‑/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro". International Journal of Molecular Medicine 43.3 (2019): 1128-1138.
Chicago
Xu, Y., Sun, P., Wang, J., Li, Z., Gao, R., Wang, X., Phillips, W. D., Liang, S. X."Differentiation of CD45‑/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro". International Journal of Molecular Medicine 43, no. 3 (2019): 1128-1138. https://doi.org/10.3892/ijmm.2019.4053