The p73 gene is less involved in the development but involved in the progression of neuroblastoma.

  • Authors:
    • H W Yang
    • H Y Piao
    • Y Z Chen
    • J Takita
    • M Kobayashi
    • M Taniwaki
    • K Hashizume
    • R Hanada
    • K Yamamoto
    • T Taki
    • F Bessho
    • M Yanagisawa
    • Y Hayashi
  • View Affiliations

  • Published online on: April 1, 2000     https://doi.org/10.3892/ijmm.5.4.379
  • Pages: 379-463
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Abstract

We performed expression, mutation, loss of heterozygosity (LOH) and fluorescence in situ hybridization (FISH) analyses of the p73 gene in neuroblastomas (NBs). Reverse transcription-polymerase chain reaction (RT-PCR) using primers which can detect both the p73alpha and p73beta transcripts was performed on 30 fresh NBs and 22 NB cell lines. Aberrant expression of the p73 gene was found in 4 (25%) of 16 primary tumors found by mass screening and in 10 (71.4%) of 14 primary tumors found clinically. The rates of expression in these two types of tumors were significantly different (p=0.026, Fisher's exact test). The incidence of aberrant expression of the p73 gene was significantly higher in stage IV patients than in stages I, II, III plus IVS patients (p=0.0236, Fisher's exact test). No homozygous deletions or rearrangements of the p73 gene were found in any samples examined. In addition to the polymorphism in exon 2, a silent mutation (codon 336 GCC/GCT) was found in one primary tumor. LOH of the p73 gene was detected in 5 (15%) of 33 primary NBs using PCR-LOH analysis. FISH analysis showed that all 17 NB cell lines used in this study revealed allelic loss of the p73 gene, while most of them expressed the p73 gene. These results suggest that the p73 gene is not monoallelically expressed in NB. We conclude that the p73 gene is less involved in the development but involved in the progression of neuroblastoma.

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Apr 2000
Volume 5 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yang H, Piao H, Chen Y, Takita J, Kobayashi M, Taniwaki M, Hashizume K, Hanada R, Yamamoto K, Taki T, Taki T, et al: The p73 gene is less involved in the development but involved in the progression of neuroblastoma.. Int J Mol Med 5: 379-463, 2000
APA
Yang, H., Piao, H., Chen, Y., Takita, J., Kobayashi, M., Taniwaki, M. ... Hayashi, Y. (2000). The p73 gene is less involved in the development but involved in the progression of neuroblastoma.. International Journal of Molecular Medicine, 5, 379-463. https://doi.org/10.3892/ijmm.5.4.379
MLA
Yang, H., Piao, H., Chen, Y., Takita, J., Kobayashi, M., Taniwaki, M., Hashizume, K., Hanada, R., Yamamoto, K., Taki, T., Bessho, F., Yanagisawa, M., Hayashi, Y."The p73 gene is less involved in the development but involved in the progression of neuroblastoma.". International Journal of Molecular Medicine 5.4 (2000): 379-463.
Chicago
Yang, H., Piao, H., Chen, Y., Takita, J., Kobayashi, M., Taniwaki, M., Hashizume, K., Hanada, R., Yamamoto, K., Taki, T., Bessho, F., Yanagisawa, M., Hayashi, Y."The p73 gene is less involved in the development but involved in the progression of neuroblastoma.". International Journal of Molecular Medicine 5, no. 4 (2000): 379-463. https://doi.org/10.3892/ijmm.5.4.379