H-ras-1 point mutation in malignant peripheral nerve sheath tumors: polymerase chain reaction restriction fragment length polymorphism analysis and direct sequencing from paraffin-embedded tissues.

  • Authors:
    • T Watanabe
    • A Sakamoto
    • S Tamiya
    • Y Oda
    • K Masuda
    • M Tsuneyoshi
  • View Affiliations

  • Published online on: June 1, 2000     https://doi.org/10.3892/ijmm.5.6.605
  • Pages: 605-613
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Abstract

It has been shown that the NF1 (neurofibromatosis type 1) gene encodes a tumor suppressor which inactivates ras proteins. Among malignant mesenchymal tumors, H-ras-1 mutations have been found in malignant fibrous histiocytoma, leiomyosarcoma and embryonal rhabdomyosarcoma. However, studies on H-ras-1 mutation of many cases of malignant peripheral nerve sheath tumors (MPNST) have not been documented. Therefore, we investigated H-ras-1 mutations of MPNST. In 45 cases of MPNSTs of our files, DNA was extracted from the formalin-fixed paraffin-embedded tissue, and the mutations of the H-ras-1 gene were detected by using PCR-RFLP (polymerase chain reaction- restriction fragment length polymorphisms) method and direct sequencing. We found two cases with H-ras-1 point mutation in MPNST for the first time. Both cases showed the same mutation in codon 13.1 [GGT(Gly) to AGT(Ser) transition]. Interestingly, both cases were associated with NF1. It is possibile that the mutation of the H-ras-1 gene occurred after the mutation of the NF1 gene in the MPNST.

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Jun 2000
Volume 5 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Watanabe T, Sakamoto A, Tamiya S, Oda Y, Masuda K and Tsuneyoshi M: H-ras-1 point mutation in malignant peripheral nerve sheath tumors: polymerase chain reaction restriction fragment length polymorphism analysis and direct sequencing from paraffin-embedded tissues.. Int J Mol Med 5: 605-613, 2000
APA
Watanabe, T., Sakamoto, A., Tamiya, S., Oda, Y., Masuda, K., & Tsuneyoshi, M. (2000). H-ras-1 point mutation in malignant peripheral nerve sheath tumors: polymerase chain reaction restriction fragment length polymorphism analysis and direct sequencing from paraffin-embedded tissues.. International Journal of Molecular Medicine, 5, 605-613. https://doi.org/10.3892/ijmm.5.6.605
MLA
Watanabe, T., Sakamoto, A., Tamiya, S., Oda, Y., Masuda, K., Tsuneyoshi, M."H-ras-1 point mutation in malignant peripheral nerve sheath tumors: polymerase chain reaction restriction fragment length polymorphism analysis and direct sequencing from paraffin-embedded tissues.". International Journal of Molecular Medicine 5.6 (2000): 605-613.
Chicago
Watanabe, T., Sakamoto, A., Tamiya, S., Oda, Y., Masuda, K., Tsuneyoshi, M."H-ras-1 point mutation in malignant peripheral nerve sheath tumors: polymerase chain reaction restriction fragment length polymorphism analysis and direct sequencing from paraffin-embedded tissues.". International Journal of Molecular Medicine 5, no. 6 (2000): 605-613. https://doi.org/10.3892/ijmm.5.6.605