Molecular alterations of E-cadherin gene: possible role in human bladder carcinogenesis.

  • Authors:
    • I Taddei
    • M Piazzini
    • R Bartoletti
    • M Dal Canto
    • I Sardi
  • View Affiliations

  • Published online on: August 1, 2000     https://doi.org/10.3892/ijmm.6.2.201
  • Pages: 201-209
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Abstract

E-cadherin is a transmembrane glycoprotein which mediates a calcium dependent homophilic interaction among epithelial cells. The altered expression and gene mutations of E-cadherin adhesion molecule have been frequently observed in various tumors. Several invasive carcinomas showed cell-cell adhesion loss although the tumor cells expressed considerable amounts of E-cadherin protein. The purpose of this study was to evaluate the role of E-cadherin gene alterations in genesis and progression of bladder carcinoma by mutation analysis of coding region, expression analysis and microsatellite instability at E-cadherin chromosome locus. We analyzed 30 bladder carcinoma (28 transitional and 2 squamous cell carcinoma) at different stage and grade. The mutation analysis showed that in one case there was a presence of a point mutation at codon 846 that consisted of a G (AGC) to C (ACC) transversion resulting in the replacement of R to T. In another sample the sequence analysis revealed a same-sense mutation at the codon 785 (AAC - AAT). The study of E-cadherin mRNA by Northern blot analysis showed that there were no differences of mRNA levels between tumor and normal mucosa samples. We noted that invasive and anaplastic tumors showed a trend to loss of expression, even if we did not find any statistically significant differences. The microsatellite analysis showed the presence of genomic instability in proximity of the E-cadherin gene. Nine out of 30 (30%) specimens presented molecular alterations in at least one out of 2 loci (D16S260 and D16S301) analyzed. The comparison between microsatellite mutations and clinical-histopathological parameters revealed a higher number of alterations in invasive respect to superficial tumors (p=0.014). On the other hand, there were no statistical differences regarding the correlation with pathological grade. These observations, which, nevertheless, need to be confirmed in a larger number of patients, suggest that alterations of E-cadherin gene may be related to pathobiology of bladder cancer development and clinical progression.

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Aug 2000
Volume 6 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Taddei I, Piazzini M, Bartoletti R, Dal Canto M and Sardi I: Molecular alterations of E-cadherin gene: possible role in human bladder carcinogenesis.. Int J Mol Med 6: 201-209, 2000
APA
Taddei, I., Piazzini, M., Bartoletti, R., Dal Canto, M., & Sardi, I. (2000). Molecular alterations of E-cadherin gene: possible role in human bladder carcinogenesis.. International Journal of Molecular Medicine, 6, 201-209. https://doi.org/10.3892/ijmm.6.2.201
MLA
Taddei, I., Piazzini, M., Bartoletti, R., Dal Canto, M., Sardi, I."Molecular alterations of E-cadherin gene: possible role in human bladder carcinogenesis.". International Journal of Molecular Medicine 6.2 (2000): 201-209.
Chicago
Taddei, I., Piazzini, M., Bartoletti, R., Dal Canto, M., Sardi, I."Molecular alterations of E-cadherin gene: possible role in human bladder carcinogenesis.". International Journal of Molecular Medicine 6, no. 2 (2000): 201-209. https://doi.org/10.3892/ijmm.6.2.201