Caspase-1 activity as a possible predictor of apoptosis induced by cisplatin in gastric cancer cells.

  • Authors:
    • K Muguruma
    • B Nakata
    • K Yanagawa
    • A Nitta
    • M Yashiro
    • N Onoda
    • K Hirakawa
  • View Affiliations

  • Published online on: November 1, 2000     https://doi.org/10.3892/ijmm.6.5.553
  • Pages: 553-560
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Abstract

Recent studies have shown that caspases, which are cystein proteases, elevate endonuclease activity and induce apoptosis. Caspase-1, an interleukin-1beta converting enzyme, has been reported to be related with anti-cancer drug induced apoptosis as well as with caspase-3. To elucidate the caspase-1 activity, which might be a predictor for the effect of chemotherapy, we examined the changes of caspase-1 activity induced after exposure to cisplatin (CDDP) in six gastric cancer cell lines. A high correlation between the 50% inhibitory concentration (IC50) and caspase-1 activity ratio was shown (r=0.83, p=0.041) (caspase-1 activity ratio: the caspase-1 activity of cells at 4 h after CDDP treatment/the caspase-1 activity of untreated cells). Further, we examined the correlation between caspase-1 activity and apoptosis induced by CDDP in two cell lines that have very different CDDP sensitivities; OCUM-2M and OCUM-2M/DDP (IC50; 0. 85+/-0.4 microg/ml and 9.0+/-1.2 microg/ml, respectively). The apoptotic index of OCUM-2M was significantly higher than that of OCUM-2M/DDP (19.8+/-3.8% vs. 4.5+/-1.2%, respectively; p=0.0005). In both cell lines, caspase-1 activity began to increase immediately after exposure to CDDP and peaked at approximately 4 h after cessation of exposure to CDDP, and gradually decreased thereafter. The caspase-1 activity of OCUM-2M was approximately 1.8-times higher than that of OCUM-2M/DDP at 4 h after exposure to CDDP. Taken together, our results indicate that evaluating the changes of caspase-1 activity after exposure to CDDP may be useful to predict apoptosis following CDDP treatment in gastric cancer cells.

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Nov 2000
Volume 6 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Muguruma K, Nakata B, Yanagawa K, Nitta A, Yashiro M, Onoda N and Hirakawa K: Caspase-1 activity as a possible predictor of apoptosis induced by cisplatin in gastric cancer cells.. Int J Mol Med 6: 553-560, 2000
APA
Muguruma, K., Nakata, B., Yanagawa, K., Nitta, A., Yashiro, M., Onoda, N., & Hirakawa, K. (2000). Caspase-1 activity as a possible predictor of apoptosis induced by cisplatin in gastric cancer cells.. International Journal of Molecular Medicine, 6, 553-560. https://doi.org/10.3892/ijmm.6.5.553
MLA
Muguruma, K., Nakata, B., Yanagawa, K., Nitta, A., Yashiro, M., Onoda, N., Hirakawa, K."Caspase-1 activity as a possible predictor of apoptosis induced by cisplatin in gastric cancer cells.". International Journal of Molecular Medicine 6.5 (2000): 553-560.
Chicago
Muguruma, K., Nakata, B., Yanagawa, K., Nitta, A., Yashiro, M., Onoda, N., Hirakawa, K."Caspase-1 activity as a possible predictor of apoptosis induced by cisplatin in gastric cancer cells.". International Journal of Molecular Medicine 6, no. 5 (2000): 553-560. https://doi.org/10.3892/ijmm.6.5.553