Multicolor spectral karyotype analysis of a transplantable human ileal carcinoid.

  • Authors:
    • H Sjögren
    • O Nilsson
    • M Behrendt
    • L Kölby
    • A M Jacobsen Levin
    • H Ahlman
    • G Stenman
  • View Affiliations

  • Published online on: December 1, 2000     https://doi.org/10.3892/ijmm.6.6.629
  • Pages: 629-662
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Abstract

In this report we present the results of a combined cytogenetic and multicolor spectral karyotype (SKY) analysis of a transplantable human ileal carcinoid (GOT1). By using SKY it was possible to identify the origin and organization of all clonal marker chromosomes and to identify cryptic translocations not detectable by conventional chromosome banding. The stemline karyotype of low passage GOT1 cells was interpreted as 43,XX, der(1)del(1)(?), inv(2)(p25q13), del(3)(p21), del(5)(q13q31), del(6)(q13), -9, -13, -15, del(16) (q22). Analysis of the GOT1 cells after about 2.5 years of propagation in nude mice allowed us to follow the in vivo progression of this tumor. Relatively few additional rearrangements had occurred during this period, indicating that the GOT1 cells are genetically stable. Most of the abnormalities detected result in loss of whole or parts of chromosomes, suggesting that loss of multiple chromosomal regions, presumably containing tumor suppressor genes, might be important genetic events in ileal carcinoids.

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Dec 2000
Volume 6 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Sjögren H, Nilsson O, Behrendt M, Kölby L, Jacobsen Levin A, Ahlman H and Stenman G: Multicolor spectral karyotype analysis of a transplantable human ileal carcinoid.. Int J Mol Med 6: 629-662, 2000
APA
Sjögren, H., Nilsson, O., Behrendt, M., Kölby, L., Jacobsen Levin, A., Ahlman, H., & Stenman, G. (2000). Multicolor spectral karyotype analysis of a transplantable human ileal carcinoid.. International Journal of Molecular Medicine, 6, 629-662. https://doi.org/10.3892/ijmm.6.6.629
MLA
Sjögren, H., Nilsson, O., Behrendt, M., Kölby, L., Jacobsen Levin, A., Ahlman, H., Stenman, G."Multicolor spectral karyotype analysis of a transplantable human ileal carcinoid.". International Journal of Molecular Medicine 6.6 (2000): 629-662.
Chicago
Sjögren, H., Nilsson, O., Behrendt, M., Kölby, L., Jacobsen Levin, A., Ahlman, H., Stenman, G."Multicolor spectral karyotype analysis of a transplantable human ileal carcinoid.". International Journal of Molecular Medicine 6, no. 6 (2000): 629-662. https://doi.org/10.3892/ijmm.6.6.629