Expression of metalloproteinases and its inhibitor in later stage of rabbit neointima development.

  • Authors:
    • H Wang
    • C Liu
    • Y Song
    • D Gordon
    • M Z Alavi
    • S Moore
  • View Affiliations

  • Published online on: January 1, 2001     https://doi.org/10.3892/ijmm.7.1.105
  • Pages: 105-117
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Abstract

Neointima formation after arterial de-endothelialization refers not only to smooth muscle cell (SMC) migration and proliferation, but also involves extracellular matrix (ECM) metabolism. Most studies regarding the role of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in neointima have focused on the early phase of vascular remodeling. In this study, we examined the expression of MMP and TIMP in rabbit aortic neointima at a relatively late stage of lesion development, between 4 and 12 weeks after initial de-endothelialization. Northern blot analysis revealed expression of steady-state MMP-9 mRNA was increased up to the 4th week and MMP-2 mRNA to the 12th week after de-endothelialization. In situ hybridization shown that MMP positive cells were predominantly distributed in arterial neointima. Expression of TIMP-1 mRNA was continuously up-regulated up to the 12th week and TIMP-1 positive cells, primarily SMCs, were also localized to the neointimal tissue. Alteration at mRNA level was accompanied by that at protein level, as assessed by SDS-PAGE zymography for MMPs and immunoblotting for TIMP-1. The profile of alteration at protein level correlated well with that at mRNA level. These data suggest that synthesis of MMPs and TIMP is a prolonged process and arterial SMC is a major source of MMP production in arterial neointima. Enhanced synthesis of MMPs and TIMPs at late stage of neointimal development may contribute to arterial ECM metabolism.

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January 2001
Volume 7 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wang H, Liu C, Song Y, Gordon D, Alavi M and Moore S: Expression of metalloproteinases and its inhibitor in later stage of rabbit neointima development.. Int J Mol Med 7: 105-117, 2001
APA
Wang, H., Liu, C., Song, Y., Gordon, D., Alavi, M., & Moore, S. (2001). Expression of metalloproteinases and its inhibitor in later stage of rabbit neointima development.. International Journal of Molecular Medicine, 7, 105-117. https://doi.org/10.3892/ijmm.7.1.105
MLA
Wang, H., Liu, C., Song, Y., Gordon, D., Alavi, M., Moore, S."Expression of metalloproteinases and its inhibitor in later stage of rabbit neointima development.". International Journal of Molecular Medicine 7.1 (2001): 105-117.
Chicago
Wang, H., Liu, C., Song, Y., Gordon, D., Alavi, M., Moore, S."Expression of metalloproteinases and its inhibitor in later stage of rabbit neointima development.". International Journal of Molecular Medicine 7, no. 1 (2001): 105-117. https://doi.org/10.3892/ijmm.7.1.105