Comparison between xanthine oxidases from buttermilk and microorganisms regarding their ability to generate reactive oxygen species

  • Authors:
    • N. Wippich
    • D. Peschke
    • E. Peschke
    • J. Holtz
    • H.-J. Bromme
  • View Affiliations

  • Published online on: February 1, 2001     https://doi.org/10.3892/ijmm.7.2.211
  • Pages: 211-216
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Xanthine oxidase (XO) forms uric acid from xanthine. It is assumed that at the same time oxygen is reduced by the XO to reactive oxygen species (ROS), mainly to ·O2- and to H2O2. Under certain conditions such ROS can be highly damaging to cellular structures. Therefore, XO was frequently used as a model system, in which the impact of ROS on cellular compounds and structures has been investigated. In this in vitro study xanthine oxidases from buttermilk and from microorganisms were compared regarding their ability to generate ROS. It could be shown that both enzymes are able to transform xanthine to uric acid but differ significantly in their reductive properties to oxygen. XO from buttermilk reduces oxygen to both ·O2- and H2O2 whereas XO from microorganisms generates H2O2, but fails to form ·O2-. Since ·O2- are involved in maintaining transition metal-mediated formation of hydroxyl radicals (·OH) from H2O2, we conclude that XO from microorganisms is therefore largely unsuitable in studies investigating just the interaction of ·O2- with other ROS on cellular compounds.

Related Articles

Journal Cover

February 2001
Volume 7 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wippich N, Peschke D, Peschke E, Holtz J and Bromme H: Comparison between xanthine oxidases from buttermilk and microorganisms regarding their ability to generate reactive oxygen species. Int J Mol Med 7: 211-216, 2001
APA
Wippich, N., Peschke, D., Peschke, E., Holtz, J., & Bromme, H. (2001). Comparison between xanthine oxidases from buttermilk and microorganisms regarding their ability to generate reactive oxygen species. International Journal of Molecular Medicine, 7, 211-216. https://doi.org/10.3892/ijmm.7.2.211
MLA
Wippich, N., Peschke, D., Peschke, E., Holtz, J., Bromme, H."Comparison between xanthine oxidases from buttermilk and microorganisms regarding their ability to generate reactive oxygen species". International Journal of Molecular Medicine 7.2 (2001): 211-216.
Chicago
Wippich, N., Peschke, D., Peschke, E., Holtz, J., Bromme, H."Comparison between xanthine oxidases from buttermilk and microorganisms regarding their ability to generate reactive oxygen species". International Journal of Molecular Medicine 7, no. 2 (2001): 211-216. https://doi.org/10.3892/ijmm.7.2.211