Assessment of B-cell mass in isolated islets exposed to D-[3H]mannoheptulose

  • Authors:
    • W. J. Malaisse
    • L. Ladriere
  • View Affiliations

  • Published online on: April 1, 2001     https://doi.org/10.3892/ijmm.7.4.405
  • Pages: 405-406
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Abstract

D-mannoheptulose was recently proposed to be transported into cells mainly at the intervention of GLUT2. In the present study, it was investigated whether advantage could be taken from such a situation to assess the contribution of insulin-producing B-cells to the total mass of isolated rat pancreatic islets. After 60 min incubation at 37°C in the presence of 8.3 mM D-glucose, the intracellular distribution space of D-[3H]mannoheptulose (0.1 mM) averaged, in islets from control and streptozotocin-induced diabetic rats, respectively 49.0±2.3 and 6.2±1.5% of the corresponding intracellular 3HOH space, all values being corrected for extracellular contamination as judged from the distribution space of [U-14C]sucrose (1.0 mM). These findings indicate that the present approach indeed allows to assess the relative contribution of B-cells to total islet mass for purpose of comparison between animals with different metabolic and/or hormonal status.

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April 2001
Volume 7 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Malaisse WJ and Malaisse WJ: Assessment of B-cell mass in isolated islets exposed to D-[3H]mannoheptulose. Int J Mol Med 7: 405-406, 2001
APA
Malaisse, W.J., & Malaisse, W.J. (2001). Assessment of B-cell mass in isolated islets exposed to D-[3H]mannoheptulose. International Journal of Molecular Medicine, 7, 405-406. https://doi.org/10.3892/ijmm.7.4.405
MLA
Malaisse, W. J., Ladriere, L."Assessment of B-cell mass in isolated islets exposed to D-[3H]mannoheptulose". International Journal of Molecular Medicine 7.4 (2001): 405-406.
Chicago
Malaisse, W. J., Ladriere, L."Assessment of B-cell mass in isolated islets exposed to D-[3H]mannoheptulose". International Journal of Molecular Medicine 7, no. 4 (2001): 405-406. https://doi.org/10.3892/ijmm.7.4.405