Uptake of 1-deoxy-1-[125I]iodo-D-mannoheptulose by different cell types: in vitro and in vivo experiments

  • Authors:
    • A. Sener
    • B. Bessieres
    • P. Courtois
    • L. Ladriere
    • K. Louchami
    • H. Jijakli
    • W. J. Malaisse
  • View Affiliations

  • Published online on: May 1, 2001     https://doi.org/10.3892/ijmm.7.5.495
  • Pages: 495-500
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Abstract

D-mannoheptulose was recently proposed as a tool to label preferentially insulin-producing cells in the pancreatic gland in the perspective of the non-invasive imaging of the endocrine pancreas. In such a perspective, we have now synthesized 1-deoxy-1-[125I]iodo-D-mannoheptulose ([125I]MH) and examined its uptake by different rat cell types. No phosphorylation of [125I]MH by bovine heart hexokinase could be detected. The apparent distribution space of [125I]MH largely exceeded that of [U-14C]sucrose, considered as an extracellular marker, in erythrocytes, parotid cells, hepatocytes, pancreatic pieces and isolated pancreatic islets. Relative to the mean intracellular distribution space of 3HOH, that of [125I]MH was not significantly different in pancreatic pieces from either normal rats or streptozotocin-induced diabetic animals (STZ rats). In pancreatic islets, the uptake of [125I]MH was decreased at low temperature, but failed to be significantly affected by cytochalasin B. Sixty min after the intravenous injection of [125I]MH, the radioactive content of selected organs displayed the following hierarchy: muscle

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May 2001
Volume 7 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Sener A, Bessieres B, Courtois P, Ladriere L, Louchami K, Jijakli H and Malaisse WJ: Uptake of 1-deoxy-1-[125I]iodo-D-mannoheptulose by different cell types: in vitro and in vivo experiments. Int J Mol Med 7: 495-500, 2001
APA
Sener, A., Bessieres, B., Courtois, P., Ladriere, L., Louchami, K., Jijakli, H., & Malaisse, W.J. (2001). Uptake of 1-deoxy-1-[125I]iodo-D-mannoheptulose by different cell types: in vitro and in vivo experiments. International Journal of Molecular Medicine, 7, 495-500. https://doi.org/10.3892/ijmm.7.5.495
MLA
Sener, A., Bessieres, B., Courtois, P., Ladriere, L., Louchami, K., Jijakli, H., Malaisse, W. J."Uptake of 1-deoxy-1-[125I]iodo-D-mannoheptulose by different cell types: in vitro and in vivo experiments". International Journal of Molecular Medicine 7.5 (2001): 495-500.
Chicago
Sener, A., Bessieres, B., Courtois, P., Ladriere, L., Louchami, K., Jijakli, H., Malaisse, W. J."Uptake of 1-deoxy-1-[125I]iodo-D-mannoheptulose by different cell types: in vitro and in vivo experiments". International Journal of Molecular Medicine 7, no. 5 (2001): 495-500. https://doi.org/10.3892/ijmm.7.5.495