Angiogenic activity of leptin in the chick embryo chorioallantoic membrane is in part mediated by endogenous fibroblast growth factor-2

  • Authors:
    • D. Ribatti
    • B. Nico
    • A. S. Belloni
    • A. Vacca
    • L. Roncali
    • G. G. Nussdorfer
  • View Affiliations

  • Published online on: September 1, 2001     https://doi.org/10.3892/ijmm.8.3.265
  • Pages: 265-268
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Abstract

Recently, it has been demonstrated that leptin, the product of the ob gene, playing a key role in the regulation of body weight, is angiogenic in vitro and in vivo. In this study we investigated the angiogenic potential of human leptin in vivo by using the chick embryo chorioallantoic membrane (CAM) assay, with the aim to establish whether this angiogenic activity is partly dependent on endogenous fibroblast growth factor-2 (FGF-2), which is normally expressed during CAM development. Results showed that leptin is able to stimulate angiogenesis and that the angiogenic response is similar to that obtained with FGF-2. The stimulating property of leptin is specific, as the application of anti-leptin antibodies onto the CAM significantly inhibits the angiogenic response. Moreover, this angiogenic activity is in part due to the activation of endogenous FGF-2. The application of anti-FGF-2 antibodies reduces the angiogenic response to leptin by 40%. Our study confirms that leptin is angiogenic in vivo and suggests that, at least in the chick CAM, its activity is in part mediated by the activation of endogenous FGF-2.

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September 2001
Volume 8 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ribatti D, Nico B, Belloni AS, Vacca A, Roncali L and Nussdorfer GG: Angiogenic activity of leptin in the chick embryo chorioallantoic membrane is in part mediated by endogenous fibroblast growth factor-2. Int J Mol Med 8: 265-268, 2001
APA
Ribatti, D., Nico, B., Belloni, A.S., Vacca, A., Roncali, L., & Nussdorfer, G.G. (2001). Angiogenic activity of leptin in the chick embryo chorioallantoic membrane is in part mediated by endogenous fibroblast growth factor-2. International Journal of Molecular Medicine, 8, 265-268. https://doi.org/10.3892/ijmm.8.3.265
MLA
Ribatti, D., Nico, B., Belloni, A. S., Vacca, A., Roncali, L., Nussdorfer, G. G."Angiogenic activity of leptin in the chick embryo chorioallantoic membrane is in part mediated by endogenous fibroblast growth factor-2". International Journal of Molecular Medicine 8.3 (2001): 265-268.
Chicago
Ribatti, D., Nico, B., Belloni, A. S., Vacca, A., Roncali, L., Nussdorfer, G. G."Angiogenic activity of leptin in the chick embryo chorioallantoic membrane is in part mediated by endogenous fibroblast growth factor-2". International Journal of Molecular Medicine 8, no. 3 (2001): 265-268. https://doi.org/10.3892/ijmm.8.3.265