Expression and interaction of different catenins in colorectal carcinoma cells

  • Authors:
    • D. Kucerova
    • E. Sloncova
    • Z. Tuhackova
    • M. Vojtechova
    • V. Sovova
  • View Affiliations

  • Published online on: December 1, 2001     https://doi.org/10.3892/ijmm.8.6.695
  • Pages: 695-698
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Aberrant signalling activities of β-catenin, originally identified as a component of cell-adhesion complexes, are now considered to be an important factor in colorectal carcinogenesis. However, recently it was shown that also γ- as well as p120 catenins have a dual role either in cell adhesion or in affecting some gene activation. Therefore, the levels and interactions of these three catenins in human colorectal carcinoma cell lines were analysed. A great heterogeneity in the expression of all catenins tested was found in colorectal carcinoma cell lines HT29 and LS174T. Detailed analysis of β-catenin interactions was done. GST-APC fragment-fused proteins were used to absorb β-catenin and its complexes from cell lysates. Similarly, the E-cadherin binding capacity of the residual pool of β-catenin was analysed using the GST-ECT construct. It was found that the level of β-catenin does not necessarily depend either on the APC or β-catenin gene mutations and that co-precipitation of β-, γ-, and p120 catenins is not limited to cells that express E-cadherin.

Related Articles

Journal Cover

December 2001
Volume 8 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Kucerova D, Sloncova E, Tuhackova Z, Vojtechova M and Sovova V: Expression and interaction of different catenins in colorectal carcinoma cells. Int J Mol Med 8: 695-698, 2001
APA
Kucerova, D., Sloncova, E., Tuhackova, Z., Vojtechova, M., & Sovova, V. (2001). Expression and interaction of different catenins in colorectal carcinoma cells. International Journal of Molecular Medicine, 8, 695-698. https://doi.org/10.3892/ijmm.8.6.695
MLA
Kucerova, D., Sloncova, E., Tuhackova, Z., Vojtechova, M., Sovova, V."Expression and interaction of different catenins in colorectal carcinoma cells". International Journal of Molecular Medicine 8.6 (2001): 695-698.
Chicago
Kucerova, D., Sloncova, E., Tuhackova, Z., Vojtechova, M., Sovova, V."Expression and interaction of different catenins in colorectal carcinoma cells". International Journal of Molecular Medicine 8, no. 6 (2001): 695-698. https://doi.org/10.3892/ijmm.8.6.695