Effects of cytochalasin B and D upon insulin release and pancreatic islet cell metabolism

  • Authors:
    • H. Jijakli
    • H.-X. Zhang
    • E. Dura
    • R. Ramirez
    • A. Sener
    • W. J. Malaisse
  • View Affiliations

  • Published online on: February 1, 2002     https://doi.org/10.3892/ijmm.9.2.165
  • Pages: 165-172
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Cytochalasin B is known to enhance insulin release evoked by nutrient and non-nutrient secretagogues, including D-glucose, despite inhibiting D-glucose uptake and metabolism in pancreatic islets. In the present study, cytochalasin D, which failed to affect D-glucose uptake and metabolism by isolated islets, also augmented glucose-stimulated insulin release, but unexpectedly to a lesser extent than cytochalasin B. Such was not the case, however, in islets stimulated by non-glucidic nutrients such as 2-ketoisocaproate or the association of L-leucine and L-glutamine. This situation coincided with the fact that cytochalasin B inhibited more severely D-glucose metabolism in non-B, as distinct from B, islet cells and, in the former case, caused a relatively greater inhibition of hexose catabolism at 2.8 mM than at 16.7 mM D-glucose. Nevertheless, even in the presence of forskolin, cytochalasin B was more efficient than cytochalasin D in augmenting glucose-stimulated insulin secretion. Thus, although these data document that non-B islet cells are more sensitive than purified islet B cells to cytochalasin B, at least in terms of inhibition of D-glucose catabolism, such a difference and its possible consequence upon the release of glucagon and other non-insulinic hormones by non-B islet cells do not appear sufficient to account for the greater enhancing action of cytochalasin B, as distinct from cytochalasin D, upon glucose-stimulated insulin output. Likewise, the latter difference does not appear attributable to a greater efficiency of cytochalasin B, as compared to cytochalasin D, upon the mechanical events involved in nutrient-stimulated exocytosis of insulin granules. Hence, the present findings suggest a so-far-unidentified interference of cytochalasin B with the B-cell glucose-sensing device.

Related Articles

Journal Cover

February 2002
Volume 9 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Jijakli H, Zhang H, Dura E, Ramirez R, Sener A and Malaisse WJ: Effects of cytochalasin B and D upon insulin release and pancreatic islet cell metabolism. Int J Mol Med 9: 165-172, 2002
APA
Jijakli, H., Zhang, H., Dura, E., Ramirez, R., Sener, A., & Malaisse, W.J. (2002). Effects of cytochalasin B and D upon insulin release and pancreatic islet cell metabolism. International Journal of Molecular Medicine, 9, 165-172. https://doi.org/10.3892/ijmm.9.2.165
MLA
Jijakli, H., Zhang, H., Dura, E., Ramirez, R., Sener, A., Malaisse, W. J."Effects of cytochalasin B and D upon insulin release and pancreatic islet cell metabolism". International Journal of Molecular Medicine 9.2 (2002): 165-172.
Chicago
Jijakli, H., Zhang, H., Dura, E., Ramirez, R., Sener, A., Malaisse, W. J."Effects of cytochalasin B and D upon insulin release and pancreatic islet cell metabolism". International Journal of Molecular Medicine 9, no. 2 (2002): 165-172. https://doi.org/10.3892/ijmm.9.2.165