Metabolism of D-[3-3H]glucose, D-[5-3H]glucose, D-[U-14C]glucose, D-[1-14C]glucose and D-[6-14C]glucose in pancreatic islets in an animal model of type-2 diabetes

  • Authors:
    • M.-H. Giroix
    • A. B. Nadi
    • A. Sener
    • B. Portha
    • W. J. Malaisse
  • View Affiliations

  • Published online on: April 1, 2002     https://doi.org/10.3892/ijmm.9.4.381
  • Pages: 381-384
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Abstract

This study aims at exploring specific aspects of D-glucose metabolism, so far not yet investigated, in pancreatic islets from adult control rats and animals (STZ rats) injected with streptozotocin during the neonatal period. The latter animals, which represent a current model of type-2 diabetes, displayed a lower body weight, higher plasma D-glucose concentration and lower insulinogenic index than control rats. The protein, DNA and insulin content were all also lower in islets prepared from STZ, rather than control rats. In the presence of 10.0 mM D-glucose, the paired ratio between D-[U-14C]glucose oxidation and D-[5-3H]glucose utilization was also decreased in the islets from STZ rats. No significant difference between control and STZ rats was observed, however, in terms of the ratios between D-[3-3H]glucose and D-[5-3H]glucose utilization, between the generation of radioactive lactate from 14C-labelled D-glucose and tritiated D-glucose utilization and between D-[1-14C]glucose and D-[6-14C]glucose oxidation. These findings reinforce the view that the previously documented preferential impairment of the oxidative modality of glycolysis in islets from STZ rats contrasts with the absence of any major anomaly in other variables of D-glucose catabolism.

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April 2002
Volume 9 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Giroix M, Nadi AB, Sener A, Portha B and Malaisse WJ: Metabolism of D-[3-3H]glucose, D-[5-3H]glucose, D-[U-14C]glucose, D-[1-14C]glucose and D-[6-14C]glucose in pancreatic islets in an animal model of type-2 diabetes. Int J Mol Med 9: 381-384, 2002
APA
Giroix, M., Nadi, A.B., Sener, A., Portha, B., & Malaisse, W.J. (2002). Metabolism of D-[3-3H]glucose, D-[5-3H]glucose, D-[U-14C]glucose, D-[1-14C]glucose and D-[6-14C]glucose in pancreatic islets in an animal model of type-2 diabetes. International Journal of Molecular Medicine, 9, 381-384. https://doi.org/10.3892/ijmm.9.4.381
MLA
Giroix, M., Nadi, A. B., Sener, A., Portha, B., Malaisse, W. J."Metabolism of D-[3-3H]glucose, D-[5-3H]glucose, D-[U-14C]glucose, D-[1-14C]glucose and D-[6-14C]glucose in pancreatic islets in an animal model of type-2 diabetes". International Journal of Molecular Medicine 9.4 (2002): 381-384.
Chicago
Giroix, M., Nadi, A. B., Sener, A., Portha, B., Malaisse, W. J."Metabolism of D-[3-3H]glucose, D-[5-3H]glucose, D-[U-14C]glucose, D-[1-14C]glucose and D-[6-14C]glucose in pancreatic islets in an animal model of type-2 diabetes". International Journal of Molecular Medicine 9, no. 4 (2002): 381-384. https://doi.org/10.3892/ijmm.9.4.381