COINDUCTION OF HSP27 PHOSPHORYLATION AND DRUG-RESISTANCE IN CHINESE-HAMSTER CELLS

  • Authors:
    • J HUOT
    • G ROY
    • H LAMBERT
    • J LANDRY
  • View Affiliations

  • Published online on: June 1, 1992     https://doi.org/10.3892/ijo.1.1.31
  • Pages: 31-36
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Abstract

Treatments of Chinese hamster fibroblasts with A23187, 2-deoxyglucose, tunicamycin, beta-mercaptoethanol or glucosamine induce an increase in cell resistance to doxorubicin and VM-26 that attains a maximum after 8 h of treatment. We tested the possibility that HSP27, a heat shock protein whose overexpression in cells was shown to confer thermoresistance and chemoresistance, might also be associated with this acquisition of drug resistance. [H-3]leucine incorporation and immunoblot analyses showed that the increased resistance was not accompanied by the synthesis or accumulation of HSP27 or other heat shock proteins. However, drug resistance promoting agents all stimulated protein kinase activities that phosphorylated HSP27 in vitro and stimulated incorporation of P-32 into HSP27 in vivo. HSP27 is thought to exert in unstressed cells functions linked with growth signal pathways. It is suggested that stimulation of HSP27 phosphorylation may modulate a protective function associated with the maintenance of signal transduction homeostasis.

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June 1992
Volume 1 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
HUOT J, ROY G, LAMBERT H and LANDRY J: COINDUCTION OF HSP27 PHOSPHORYLATION AND DRUG-RESISTANCE IN CHINESE-HAMSTER CELLS. Int J Oncol 1: 31-36, 1992
APA
HUOT, J., ROY, G., LAMBERT, H., & LANDRY, J. (1992). COINDUCTION OF HSP27 PHOSPHORYLATION AND DRUG-RESISTANCE IN CHINESE-HAMSTER CELLS. International Journal of Oncology, 1, 31-36. https://doi.org/10.3892/ijo.1.1.31
MLA
HUOT, J., ROY, G., LAMBERT, H., LANDRY, J."COINDUCTION OF HSP27 PHOSPHORYLATION AND DRUG-RESISTANCE IN CHINESE-HAMSTER CELLS". International Journal of Oncology 1.1 (1992): 31-36.
Chicago
HUOT, J., ROY, G., LAMBERT, H., LANDRY, J."COINDUCTION OF HSP27 PHOSPHORYLATION AND DRUG-RESISTANCE IN CHINESE-HAMSTER CELLS". International Journal of Oncology 1, no. 1 (1992): 31-36. https://doi.org/10.3892/ijo.1.1.31