HUMAN NM23-H1-PROTEIN AND H2-PROTEIN HAVE SIMILAR NUCLEOSIDE DIPHOSPHATE KINASE-ACTIVITIES

  • Authors:
    • T URANO
    • S FUSHIDA
    • K FURUKAWA
    • H SHIKU
  • View Affiliations

  • Published online on: September 1, 1992     https://doi.org/10.3892/ijo.1.4.425
  • Pages: 425-430
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Abstract

The nucleoside diphosphate kinase activity of nm23-H1 and nm23-H2 proteins was examined. Full length nm23-H1 and nm23-H2 proteins were produced in E.coli in fusion form with a 26 kDa glutathione S-transferase (GST). Affinity purified nm23-H1 and nm23-H2 formed phosphoenzyme intermediates when incubated with [gamma-P-32]ATP. The formation of GTP from GDP was also demonstrated by these two proteins by thin layer chromatography. The 26 kDa GST alone did not show similar activity. Both nm23-H1 and nm23-H2 shared very similar biochemical characteristics, namely, time kinetics, pH, temperature and cation dependency for the formation of the phosphoenzyme intermediates.

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September 1992
Volume 1 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
URANO T, FUSHIDA S, FURUKAWA K and SHIKU H: HUMAN NM23-H1-PROTEIN AND H2-PROTEIN HAVE SIMILAR NUCLEOSIDE DIPHOSPHATE KINASE-ACTIVITIES. Int J Oncol 1: 425-430, 1992
APA
URANO, T., FUSHIDA, S., FURUKAWA, K., & SHIKU, H. (1992). HUMAN NM23-H1-PROTEIN AND H2-PROTEIN HAVE SIMILAR NUCLEOSIDE DIPHOSPHATE KINASE-ACTIVITIES. International Journal of Oncology, 1, 425-430. https://doi.org/10.3892/ijo.1.4.425
MLA
URANO, T., FUSHIDA, S., FURUKAWA, K., SHIKU, H."HUMAN NM23-H1-PROTEIN AND H2-PROTEIN HAVE SIMILAR NUCLEOSIDE DIPHOSPHATE KINASE-ACTIVITIES". International Journal of Oncology 1.4 (1992): 425-430.
Chicago
URANO, T., FUSHIDA, S., FURUKAWA, K., SHIKU, H."HUMAN NM23-H1-PROTEIN AND H2-PROTEIN HAVE SIMILAR NUCLEOSIDE DIPHOSPHATE KINASE-ACTIVITIES". International Journal of Oncology 1, no. 4 (1992): 425-430. https://doi.org/10.3892/ijo.1.4.425