Purification and characterisation of soluble intercellular adhesion molecule-1 (sICAM-1) and its effect on cell-mediated cytolysis of tumour cells

  • Authors:
    • D Young
    • A Jackson
    • K James
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  • Published online on: April 1, 1997     https://doi.org/10.3892/ijo.10.4.827
  • Pages: 827-834
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Abstract

The aim of this study was to determine the effects of highly purified soluble ICAM-1 (sICAM-1) from a variety of sources on lymphokine-activated killer (LAK) cell-mediated cytolysis of bladder tumour cells. Soluble ICAM-1 was isolated by immunoaffinity chromatography using two different anti-ICAM-1 monoclonal antibody (mAb) columns from normal human serum, bladder tumour cell culture supernatants and the urine of patients receiving bacillus Calmette-Guerin (BCG) immunotherapy for bladder cancer. Soluble ICAM-1 from all sources resolved as a single diffuse band with a molecular weight of 80 to 90 kilodaltons (kDa) on Western blots under both non-reducing and reducing conditions, consistent with the predicted molecular weight for monomeric sICAM-1. Monomeric sICAM-1 isolated from serum retained its ability to inhibit the binding of an anti-ICAM-1 mAb to ICAM-1 positive target cells. In contrast, monomeric sICAM-1 isolated from serum and urine failed to inhibit LAK cell-mediated cytolysis of four bladder tumour cell lines. These results are in agreement with recent observations that the monomeric form of sICAM-1 binds to its receptor LFA-1 with extremely low affinity, indicating that at physiological concentrations sICAM-1 does not interfere in ICAM-1/LFA-1 mediated cellular adhesion events.

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April 1997
Volume 10 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Young D, Jackson A and James K: Purification and characterisation of soluble intercellular adhesion molecule-1 (sICAM-1) and its effect on cell-mediated cytolysis of tumour cells. Int J Oncol 10: 827-834, 1997
APA
Young, D., Jackson, A., & James, K. (1997). Purification and characterisation of soluble intercellular adhesion molecule-1 (sICAM-1) and its effect on cell-mediated cytolysis of tumour cells. International Journal of Oncology, 10, 827-834. https://doi.org/10.3892/ijo.10.4.827
MLA
Young, D., Jackson, A., James, K."Purification and characterisation of soluble intercellular adhesion molecule-1 (sICAM-1) and its effect on cell-mediated cytolysis of tumour cells". International Journal of Oncology 10.4 (1997): 827-834.
Chicago
Young, D., Jackson, A., James, K."Purification and characterisation of soluble intercellular adhesion molecule-1 (sICAM-1) and its effect on cell-mediated cytolysis of tumour cells". International Journal of Oncology 10, no. 4 (1997): 827-834. https://doi.org/10.3892/ijo.10.4.827