Altered biochemical profile and gene expression in aflatoxin B-1-transformed C3H10T1/2 cells

  • Authors:
    • S Nadadur
    • K Lisciandro
    • A Mudipalli
    • A Maccubbin
    • M Faletto
    • H Gurtoo
  • View Affiliations

  • Published online on: June 1, 1997     https://doi.org/10.3892/ijo.10.6.1265
  • Pages: 1265-1275
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Abstract

A transformed cell line 7SA, obtained by transformation of C3H10T1/2 cells with irt vitro activated aflatoxin B-1 (AFB(1)), was used to investigate biochemical and molecular alterations associated with transformation by AFB(1). 7SA cells demonstrate an altered biochemical phenotype characterized by alterations in phase I and phase II enzymes in a manner that would allow these cells to survive in a hostile chemical environment. Investigations of the molecular basis of transformation revealed no mutations in codons 12/13 and 61 of ras genes (Ha-, Ki- and N-ras) and in exons 5, 6, 7 and 8 of p53 tumor suppressor gene. However, subtractive hybridization led to the isolation of seven novel cDNA clones that demonstrated 2 to 10-fold overexpression of the mRNAs corresponding to the five cDNAs (SK1, SK2, SK3, SK4 and SK5) and >400 fold overexpression of the mRNAs corresponding to the other two cDNAs (SK67 and SK153). In addition, part of the sequence of the cDNA clone SK5 demonstrated >88% identity with L1-like mobile genetic element and Southern analysis of the DNA with SK5 cDNA as a probe revealed gene rearrangement in 7SA DNA, compared to DNA from C3H10T1/2 cells.

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June 1997
Volume 10 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Nadadur S, Lisciandro K, Mudipalli A, Maccubbin A, Faletto M and Gurtoo H: Altered biochemical profile and gene expression in aflatoxin B-1-transformed C3H10T1/2 cells. Int J Oncol 10: 1265-1275, 1997
APA
Nadadur, S., Lisciandro, K., Mudipalli, A., Maccubbin, A., Faletto, M., & Gurtoo, H. (1997). Altered biochemical profile and gene expression in aflatoxin B-1-transformed C3H10T1/2 cells. International Journal of Oncology, 10, 1265-1275. https://doi.org/10.3892/ijo.10.6.1265
MLA
Nadadur, S., Lisciandro, K., Mudipalli, A., Maccubbin, A., Faletto, M., Gurtoo, H."Altered biochemical profile and gene expression in aflatoxin B-1-transformed C3H10T1/2 cells". International Journal of Oncology 10.6 (1997): 1265-1275.
Chicago
Nadadur, S., Lisciandro, K., Mudipalli, A., Maccubbin, A., Faletto, M., Gurtoo, H."Altered biochemical profile and gene expression in aflatoxin B-1-transformed C3H10T1/2 cells". International Journal of Oncology 10, no. 6 (1997): 1265-1275. https://doi.org/10.3892/ijo.10.6.1265