Study of nm23 gene expression in an N-methyl-N-nitrosourea transformed human breast epithelial cell line

  • Authors:
    • G Lazzaro
    • S Sharpless
    • W Qing
    • R Mehta
    • T DasGupta
  • View Affiliations

  • Published online on: October 1, 1997     https://doi.org/10.3892/ijo.11.4.765
  • Pages: 765-769
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Abstract

In the present study we pinpoint the time of nm23 down-regulation during the chemical transformation of the human breast epithelial cell line HBL100. The non-malignant HBL100 was transformed by exposing it to N-methyl-N-nitrosourea (MNU). We subsequently injected the transformed cells (HBL-T-MNU) into nude mice, resulting in tumor growth. With a second passage of these tumors in mice we observed lung and extra-regional node metastases. The expression of nm23 in the non-tumorigenic HBL100 cells was compared to the tumorigenic KBL-T-MNU cells as well as to the metastatic cell line HBL-T2(MNU) derived from the tumor induced by HBL-T-MNU cells. By using immunohistochemistry and Western blot analysis we documented the downregulation of nm23 expression in the tumorigenic HBL-T-MNU and metastatic HBL-T2(MNU) cell lines, as compared to the parental line HBL100. Once downregulated, nm23 expression in this model remains constant during the subsequent progression. These results suggest that nm23 down-regulation may indeed be associated with early neoplastic transformation and is maintained throughout neoplastic progression and metastatic stage.

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October 1997
Volume 11 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Lazzaro G, Sharpless S, Qing W, Mehta R and DasGupta T: Study of nm23 gene expression in an N-methyl-N-nitrosourea transformed human breast epithelial cell line. Int J Oncol 11: 765-769, 1997
APA
Lazzaro, G., Sharpless, S., Qing, W., Mehta, R., & DasGupta, T. (1997). Study of nm23 gene expression in an N-methyl-N-nitrosourea transformed human breast epithelial cell line. International Journal of Oncology, 11, 765-769. https://doi.org/10.3892/ijo.11.4.765
MLA
Lazzaro, G., Sharpless, S., Qing, W., Mehta, R., DasGupta, T."Study of nm23 gene expression in an N-methyl-N-nitrosourea transformed human breast epithelial cell line". International Journal of Oncology 11.4 (1997): 765-769.
Chicago
Lazzaro, G., Sharpless, S., Qing, W., Mehta, R., DasGupta, T."Study of nm23 gene expression in an N-methyl-N-nitrosourea transformed human breast epithelial cell line". International Journal of Oncology 11, no. 4 (1997): 765-769. https://doi.org/10.3892/ijo.11.4.765