The tumorigenic phenotype of a mutated form of Fc gamma RIIB1, lacking the ability to generate soluble receptor and allowing a low-level of ligand binding

  • Authors:
    • M Ran
    • T Zusman
    • E Lisansky
    • M Eskenasy
    • R Eshel
    • Y Avivi
    • Z Indik
    • A Schreiber
  • View Affiliations

  • Published online on: October 1, 1997     https://doi.org/10.3892/ijo.11.4.857
  • Pages: 857-861
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Abstract

Non immunohematopoietic murine tumor cells ectopically expressing Fc gamma RIIB1 (B1) were recently shown to express a higher tumorigenicity phenotype than cells not expressing this receptor. Utilizing a genetic approach we studied the possible contribution of a soluble form of B1 to tumor enhancement. A mutated form of the B1, lacking the cleavage site responsible for the generation of soluble B1 was produced using gene splicing by overlap extension PCR. A deletion confirmed by sequence analysis from 172 to 178 residues was generated. Stable transfectants expressed the B1 deleted form (B1 Delta) both as specific RNA and as a membrane protein receptor allowing a low level of ligand binding. The soluble form of B1 was undetectable in tissue culture supernatants of Bib transfected cells while it was present in supernatants of wild type B1-transfectants. Stable B1 Delta transfectants were significantly more tumorigenic than negative control transfectants. Tumor incidence was almost as high as that of intact B1 and lagged in the latency period before the appearance of palpable tumors. It is suggested that the soluble B1 has a minimal contribution to tumor enhancement.

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October 1997
Volume 11 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Ran M, Zusman T, Lisansky E, Eskenasy M, Eshel R, Avivi Y, Indik Z and Schreiber A: The tumorigenic phenotype of a mutated form of Fc gamma RIIB1, lacking the ability to generate soluble receptor and allowing a low-level of ligand binding. Int J Oncol 11: 857-861, 1997
APA
Ran, M., Zusman, T., Lisansky, E., Eskenasy, M., Eshel, R., Avivi, Y. ... Schreiber, A. (1997). The tumorigenic phenotype of a mutated form of Fc gamma RIIB1, lacking the ability to generate soluble receptor and allowing a low-level of ligand binding. International Journal of Oncology, 11, 857-861. https://doi.org/10.3892/ijo.11.4.857
MLA
Ran, M., Zusman, T., Lisansky, E., Eskenasy, M., Eshel, R., Avivi, Y., Indik, Z., Schreiber, A."The tumorigenic phenotype of a mutated form of Fc gamma RIIB1, lacking the ability to generate soluble receptor and allowing a low-level of ligand binding". International Journal of Oncology 11.4 (1997): 857-861.
Chicago
Ran, M., Zusman, T., Lisansky, E., Eskenasy, M., Eshel, R., Avivi, Y., Indik, Z., Schreiber, A."The tumorigenic phenotype of a mutated form of Fc gamma RIIB1, lacking the ability to generate soluble receptor and allowing a low-level of ligand binding". International Journal of Oncology 11, no. 4 (1997): 857-861. https://doi.org/10.3892/ijo.11.4.857