Expression and synthesis of insulin-like growth factor-binding proteins in human glioma cell lines.

  • Authors:
    • W Zumkeller
    • D Müller
    • S Müller
    • C Günther
    • M Westphal
  • View Affiliations

  • Published online on: January 1, 1998     https://doi.org/10.3892/ijo.12.1.129
  • Pages: 129-164
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Abstract

We previously demonstrated the presence of insulin-like growth factors (IGFs) and IGF-receptors in human glioma cell lines derived from primary glioblastomas. The biological action of IGFs is modulated by specific IGF-binding proteins (IGFBP)-1 to -7. By means of polymerase chain reaction (PCR), we detected mRNA transcripts for IGFBP-1 in 42%, IGFBP-2 in 65%, IGFBP-3 in 97%, IGFBP-4 in 3%, IGFBP-5 in 74%, IGFBP-6 in 94% and IGFBP-7 in 87% of the glioma cell lines. The specificity of the PCR reaction was verified by direct sequencing of the PCR product. In addition, the content of the most prevalent IGFBP-3 was measured in conditioned medium from glioma cells by specific radioimmunoassay with levels ranging from < 1 to 620 ng/ml. Moreover, the presence of membrane-bound IGFBPs (44, 50 and 60 kDa) as well as IGF-II receptors was demonstrated by using 125I-labelled IGF-II as a ligand. In conclusion, IGFBPs may modulate the IGF-mediated effects in these cell lines.

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Jan 1998
Volume 12 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Zumkeller W, Müller D, Müller S, Günther C and Westphal M: Expression and synthesis of insulin-like growth factor-binding proteins in human glioma cell lines.. Int J Oncol 12: 129-164, 1998
APA
Zumkeller, W., Müller, D., Müller, S., Günther, C., & Westphal, M. (1998). Expression and synthesis of insulin-like growth factor-binding proteins in human glioma cell lines.. International Journal of Oncology, 12, 129-164. https://doi.org/10.3892/ijo.12.1.129
MLA
Zumkeller, W., Müller, D., Müller, S., Günther, C., Westphal, M."Expression and synthesis of insulin-like growth factor-binding proteins in human glioma cell lines.". International Journal of Oncology 12.1 (1998): 129-164.
Chicago
Zumkeller, W., Müller, D., Müller, S., Günther, C., Westphal, M."Expression and synthesis of insulin-like growth factor-binding proteins in human glioma cell lines.". International Journal of Oncology 12, no. 1 (1998): 129-164. https://doi.org/10.3892/ijo.12.1.129