Possible involvement of calcineurin in retinoic acid-induced inhibition of leukemic HL-60 cell proliferation.

  • Authors:
    • H Kihira
    • A Hiasa
    • M Yamamoto
    • N Katayama
    • T Kuno
    • K Ohtsuka
    • H Shiku
    • M Nishikawa
  • View Affiliations

  • Published online on: March 1, 1998     https://doi.org/10.3892/ijo.12.3.629
  • Pages: 629-663
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Abstract

Differentiation of leukemic HL-60 cells by all transretinoic acid (ATRA) resulted in a reduced rate of growth. Cyclosporin A and FK506, at concentrations that inhibited calcineurin activity, abrogated the ATRA-induced inhibition of HL-60 cell growth but these immunosuppressants had no effect on the ATRA-induced granulocytic differentiation. Treatment with 1 microM ATRA led to a progressive increase in calcineurin phosphatase activity of HL-60 cells; the increase in this activity appeared to parallel the functional change of HL-60 cells during granulocytic differentiation. Increase in calcineurin activity was concordant with the increased expressions of calcineurin A and calcineurin B subunit proteins. The FKBP12 expression increased during ATRA-induced differentiation and expression of cyclophilin A remained unchanged. We propose that the increased expression of calcineurin is involved in the ATRA-induced inhibition of HL-60 cell proliferation, as in the case with 1,25alpha-dihydroxy-vitamin D3.

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Mar 1998
Volume 12 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kihira H, Hiasa A, Yamamoto M, Katayama N, Kuno T, Ohtsuka K, Shiku H and Nishikawa M: Possible involvement of calcineurin in retinoic acid-induced inhibition of leukemic HL-60 cell proliferation.. Int J Oncol 12: 629-663, 1998
APA
Kihira, H., Hiasa, A., Yamamoto, M., Katayama, N., Kuno, T., Ohtsuka, K. ... Nishikawa, M. (1998). Possible involvement of calcineurin in retinoic acid-induced inhibition of leukemic HL-60 cell proliferation.. International Journal of Oncology, 12, 629-663. https://doi.org/10.3892/ijo.12.3.629
MLA
Kihira, H., Hiasa, A., Yamamoto, M., Katayama, N., Kuno, T., Ohtsuka, K., Shiku, H., Nishikawa, M."Possible involvement of calcineurin in retinoic acid-induced inhibition of leukemic HL-60 cell proliferation.". International Journal of Oncology 12.3 (1998): 629-663.
Chicago
Kihira, H., Hiasa, A., Yamamoto, M., Katayama, N., Kuno, T., Ohtsuka, K., Shiku, H., Nishikawa, M."Possible involvement of calcineurin in retinoic acid-induced inhibition of leukemic HL-60 cell proliferation.". International Journal of Oncology 12, no. 3 (1998): 629-663. https://doi.org/10.3892/ijo.12.3.629