Potentiation of tumor necrosis factor induced apoptosis by onconase.

  • Authors:
    • A Deptala
    • H D Halicka
    • B Ardelt
    • W Ardelt
    • S M Mikulski
    • K Shogen
    • Z Darzynkiewicz
  • View Affiliations

  • Published online on: July 1, 1998     https://doi.org/10.3892/ijo.13.1.11
  • Pages: 11-17
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Abstract

Onconase (ONC) a ribonuclease from amphibian oocytes is cytostatic and cytotoxic to many human tumor lines, shows in vivo antitumor activity in mouse tumor models and is in Phase III clinical trials. The mechanism of antitumor activity of ONC is presumed to be due to its internalization, degradation of intracellular RNA and suppression of protein synthesis. Since apoptosis triggered by TNF-alpha is known to be potentiated by inhibitors of protein synthesis, we have hypothesized that it also may be potentiated by ONC. Indeed, preincubation of U-937 or HL-60 leukemic cells with 0.17 microM ONC rendered them more sensitive to induction of apoptosis by TNF-alpha or antibody to CD95 (Fas). The mechanism by which ONC amplifies the effect of TNF-alpha may involve suppression of induction of the survival genes whose expression is triggered by activation of NFkB by this factor.

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Jul 1998
Volume 13 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Deptala A, Halicka H, Ardelt B, Ardelt W, Mikulski S, Shogen K and Darzynkiewicz Z: Potentiation of tumor necrosis factor induced apoptosis by onconase.. Int J Oncol 13: 11-17, 1998
APA
Deptala, A., Halicka, H., Ardelt, B., Ardelt, W., Mikulski, S., Shogen, K., & Darzynkiewicz, Z. (1998). Potentiation of tumor necrosis factor induced apoptosis by onconase.. International Journal of Oncology, 13, 11-17. https://doi.org/10.3892/ijo.13.1.11
MLA
Deptala, A., Halicka, H., Ardelt, B., Ardelt, W., Mikulski, S., Shogen, K., Darzynkiewicz, Z."Potentiation of tumor necrosis factor induced apoptosis by onconase.". International Journal of Oncology 13.1 (1998): 11-17.
Chicago
Deptala, A., Halicka, H., Ardelt, B., Ardelt, W., Mikulski, S., Shogen, K., Darzynkiewicz, Z."Potentiation of tumor necrosis factor induced apoptosis by onconase.". International Journal of Oncology 13, no. 1 (1998): 11-17. https://doi.org/10.3892/ijo.13.1.11