Antitumor vaccine effect of irradiated murine neuroblastoma cells producing interleukin-2 or granulocyte macrophage-colony stimulating factor.

  • Authors:
    • H Yoshida
    • H Enomoto
    • K Kawamura
    • K Takenaga
    • M Tanabe
    • N Ohnuma
    • S Sakiyama
    • M Tagawa
  • View Affiliations

  • Published online on: July 1, 1998     https://doi.org/10.3892/ijo.13.1.73
  • Pages: 73-81
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Abstract

We have examined vaccination effects of cytokine-producing murine neuroblastoma cells (C1300). C1300 cells retrovirally transduced with interleukin-2 (IL-2) or granulocyte macrophage-colony stimulation factor (GM-CSF) gene were established. Their in vitro proliferation rates and the class I expression of major histocompatibility complex were not different from those of wild-type cells. Five-Gy irradiation of the respective cytokine producers slightly reduced the in vitro cell growth but treatment with 15 Gy significantly impaired the proliferation. In contrast, the secretion of both cytokines from the respective transduced cells was retained compared with the cell growth. We immunized syngeneic mice with irradiated wild-type cells as a control or cytokine-producing cells and challenged the mice with unirradiated wild-type cells. The control mice developed tumors of the challenged wild-type cells, on the contrary, the mice which had received irradiated IL-2 or GM-CSF producers did not. Thus, IL-2- or GM-CSF-expressing syngeneic tumor cells can be potentially used as a tumor vaccine by inducing protective immunity against low immunogenic neuroblastomas in the inoculated hosts.

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Jul 1998
Volume 13 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Yoshida H, Enomoto H, Kawamura K, Takenaga K, Tanabe M, Ohnuma N, Sakiyama S and Tagawa M: Antitumor vaccine effect of irradiated murine neuroblastoma cells producing interleukin-2 or granulocyte macrophage-colony stimulating factor.. Int J Oncol 13: 73-81, 1998
APA
Yoshida, H., Enomoto, H., Kawamura, K., Takenaga, K., Tanabe, M., Ohnuma, N. ... Tagawa, M. (1998). Antitumor vaccine effect of irradiated murine neuroblastoma cells producing interleukin-2 or granulocyte macrophage-colony stimulating factor.. International Journal of Oncology, 13, 73-81. https://doi.org/10.3892/ijo.13.1.73
MLA
Yoshida, H., Enomoto, H., Kawamura, K., Takenaga, K., Tanabe, M., Ohnuma, N., Sakiyama, S., Tagawa, M."Antitumor vaccine effect of irradiated murine neuroblastoma cells producing interleukin-2 or granulocyte macrophage-colony stimulating factor.". International Journal of Oncology 13.1 (1998): 73-81.
Chicago
Yoshida, H., Enomoto, H., Kawamura, K., Takenaga, K., Tanabe, M., Ohnuma, N., Sakiyama, S., Tagawa, M."Antitumor vaccine effect of irradiated murine neuroblastoma cells producing interleukin-2 or granulocyte macrophage-colony stimulating factor.". International Journal of Oncology 13, no. 1 (1998): 73-81. https://doi.org/10.3892/ijo.13.1.73