Activity of human papillomavirus type 16 P97 promoter in immortal and tumorigenic human oral keratinocytes.

  • Authors:
    • J K Kook
    • J H Kim
    • B M Min
  • View Affiliations

  • Published online on: October 1, 1998     https://doi.org/10.3892/ijo.13.4.765
  • Pages: 765-836
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Abstract

We previously immortalized normal human oral keratinocytes (NHOK) by transfection with cloned human papillomavirus type 16 (HPV-16) genome and converted these immortalized cells to tumorigenic cells with chemical carcinogens. Since the tumorigenic cells expressed higher level of HPV-16 E6/E7 transcripts, we predicted that enhanced E6/E7 expression was induced by mutations at the long control region (LCR) of the viral genome integrated into cellular chromosome. To test this possibility, we sequenced the entire HPV-16 LCR from immortalized and tumorigenic cells, but no difference in the sequences in all of the tested cells was observed. However, it is possible that such differences in the expression of E6/E7 could have originated from different activities of cellular transcription factors in the different cells. To examine this prospect, we subcloned entire LCR into a reporter gene and determined the promoter activity of LCR in immortalized and tumorigenic cells. We found that the LCR promoter activity was significantly higher in tumorigenic cells when comparing to immortalized cells. We also observed that at least 477 nucleotides upstream of E6 open reading frame are needed for the maximum LCR promoter activity in tumorigenic cells.

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Oct 1998
Volume 13 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kook J, Kim J and Min B: Activity of human papillomavirus type 16 P97 promoter in immortal and tumorigenic human oral keratinocytes.. Int J Oncol 13: 765-836, 1998
APA
Kook, J., Kim, J., & Min, B. (1998). Activity of human papillomavirus type 16 P97 promoter in immortal and tumorigenic human oral keratinocytes.. International Journal of Oncology, 13, 765-836. https://doi.org/10.3892/ijo.13.4.765
MLA
Kook, J., Kim, J., Min, B."Activity of human papillomavirus type 16 P97 promoter in immortal and tumorigenic human oral keratinocytes.". International Journal of Oncology 13.4 (1998): 765-836.
Chicago
Kook, J., Kim, J., Min, B."Activity of human papillomavirus type 16 P97 promoter in immortal and tumorigenic human oral keratinocytes.". International Journal of Oncology 13, no. 4 (1998): 765-836. https://doi.org/10.3892/ijo.13.4.765