Induction of differentiation and apoptosis in the prostate cancer cell line LNCaP by sodium butyrate and galectin-1.

  • Authors:
    • J Ellerhorst
    • T Nguyen
    • D N Cooper
    • Y Estrov
    • D Lotan
    • R Lotan
  • View Affiliations

  • Published online on: February 1, 1999     https://doi.org/10.3892/ijo.14.2.225
  • Pages: 225-257
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Abstract

Galectin-1 has been implicated in the process of vertebrate developmental regulation. Sodium butyrate is an established differentiation-inducing agent and has been shown to increase galectin-1 expression in colon carcinoma cells. We studied the roles of butyrate and galectin-1 in the induction of differentiation and apoptosis in the prostate cancer cell line LNCaP. Treatment of LNCaP cells with butyrate resulted in induction of galectin-1 expression in a time- and dose-dependent manner. Treatment with butyrate also resulted in inhibition of proliferation, morphologic changes consistent with a differentiated phenotype, and induction of apoptosis. Prostate specific antigen expression was transiently reduced. To determine which of these effects might be secondary to the induction of galectin-1, LNCaP cells were transfected with a galectin-1 expression vector. The transfected cells displayed growth inhibition and an increased rate of apoptosis. PSA expression was not affected. We conclude that galectin-1 may be responsible for many of the phenotypic changes resulting from butyrate treatment and may function downstream in the pathway of butyrate-induced differentiation. We also found PSA to be somewhat inconsistent as an indicator of differentiation of LNCaP cells, likely due to other factors influencing its expression.

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Feb 1999
Volume 14 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Ellerhorst J, Nguyen T, Cooper D, Estrov Y, Lotan D and Lotan R: Induction of differentiation and apoptosis in the prostate cancer cell line LNCaP by sodium butyrate and galectin-1.. Int J Oncol 14: 225-257, 1999
APA
Ellerhorst, J., Nguyen, T., Cooper, D., Estrov, Y., Lotan, D., & Lotan, R. (1999). Induction of differentiation and apoptosis in the prostate cancer cell line LNCaP by sodium butyrate and galectin-1.. International Journal of Oncology, 14, 225-257. https://doi.org/10.3892/ijo.14.2.225
MLA
Ellerhorst, J., Nguyen, T., Cooper, D., Estrov, Y., Lotan, D., Lotan, R."Induction of differentiation and apoptosis in the prostate cancer cell line LNCaP by sodium butyrate and galectin-1.". International Journal of Oncology 14.2 (1999): 225-257.
Chicago
Ellerhorst, J., Nguyen, T., Cooper, D., Estrov, Y., Lotan, D., Lotan, R."Induction of differentiation and apoptosis in the prostate cancer cell line LNCaP by sodium butyrate and galectin-1.". International Journal of Oncology 14, no. 2 (1999): 225-257. https://doi.org/10.3892/ijo.14.2.225