Differential expression of stathmin during neoplastic conversion of human prostate epithelial cells is reversed by hypomethylating agent, 5-azacytidine.

  • Authors:
    • S C Prasad
    • P J Thraves
    • V A Soldatenkov
    • S Varghese
    • A Dritschilo
  • View Affiliations

  • Published online on: March 1, 1999     https://doi.org/10.3892/ijo.14.3.529
  • Pages: 529-563
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Abstract

In a variety of human tumor tissues, including those of prostate and breast, CpG hypermethylation represents one of the mechanisms downregulating the expression of specific proteins, including tumor suppressor proteins. Using 267B1-XR cells generated by ionizing radiation-induced transformation of epithelial cells, derived from neonatal human prostate and immortalized by SV40 (267B1), we now report markedly low levels of expression of the cytoplasmic phosphoprotein stathmin, in addition to several proteins of the actin microfilaments and intermediate filaments that characterize the altered phenotype. Stathmin is emerging as a relay protein integrating signals from diverse pathways during differentiation and neoplastic progression. In this in vitro prostate carcinogenesis model system, where loss of specific-protein expression is a major feature of the transformed 267B1-XR cells, we employed 5-azacytidine treatment followed by 2D-PAGE to reveal if experimental genomic hypomethylation reinstated the levels of any of the differentially expressed proteins. Our data suggest that stathmin represents one such example.

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Mar 1999
Volume 14 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Prasad S, Thraves P, Soldatenkov V, Varghese S and Dritschilo A: Differential expression of stathmin during neoplastic conversion of human prostate epithelial cells is reversed by hypomethylating agent, 5-azacytidine.. Int J Oncol 14: 529-563, 1999
APA
Prasad, S., Thraves, P., Soldatenkov, V., Varghese, S., & Dritschilo, A. (1999). Differential expression of stathmin during neoplastic conversion of human prostate epithelial cells is reversed by hypomethylating agent, 5-azacytidine.. International Journal of Oncology, 14, 529-563. https://doi.org/10.3892/ijo.14.3.529
MLA
Prasad, S., Thraves, P., Soldatenkov, V., Varghese, S., Dritschilo, A."Differential expression of stathmin during neoplastic conversion of human prostate epithelial cells is reversed by hypomethylating agent, 5-azacytidine.". International Journal of Oncology 14.3 (1999): 529-563.
Chicago
Prasad, S., Thraves, P., Soldatenkov, V., Varghese, S., Dritschilo, A."Differential expression of stathmin during neoplastic conversion of human prostate epithelial cells is reversed by hypomethylating agent, 5-azacytidine.". International Journal of Oncology 14, no. 3 (1999): 529-563. https://doi.org/10.3892/ijo.14.3.529