Establishment and characterization of a new human hepatocellular carcinoma cell line, HAK-3, and its response to growth factors.

  • Authors:
    • I Utsunomiya
    • A Iemura
    • H Yano
    • J Akiba
    • M Kojiro
  • View Affiliations

  • Published online on: October 1, 1999     https://doi.org/10.3892/ijo.15.4.669
  • Pages: 669-744
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

A new human hepatocellular (HCC) cell line, HAK-3, was established from a resected HCC of a Japanese, female patient. HAK-3 retains morphologic features of the original HCC, and proliferates in a monolayered sheet (doubling time: 26 h). HAK-3 is a single aneuploid cell population with a DNA index of 2.42, the karyotype is human, chromosomes are 80-85 (mode: 83), and secretes fibronectin and tissue polypeptide antigen. Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) dose-dependently accelerated the cell proliferation, while deletion-type hepatocyte growth factor (dHGF) tended to suppress the proliferation, and transforming growth factor (TGF)-alpha showed almost no influence. dHGF induced the decrease of cell adhesiveness, changed the cell morphology to spindle-shaped cells, increased cell movement, and showed chemotactic effects with the increase of its concentration gradient in cultures. HAK-3 would be useful in studies on the acceleration mechanisms of cancer cell proliferation by growth factors and of chemotaxis by dHGF.

Related Articles

Journal Cover

Oct 1999
Volume 15 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Utsunomiya I, Iemura A, Yano H, Akiba J and Kojiro M: Establishment and characterization of a new human hepatocellular carcinoma cell line, HAK-3, and its response to growth factors.. Int J Oncol 15: 669-744, 1999
APA
Utsunomiya, I., Iemura, A., Yano, H., Akiba, J., & Kojiro, M. (1999). Establishment and characterization of a new human hepatocellular carcinoma cell line, HAK-3, and its response to growth factors.. International Journal of Oncology, 15, 669-744. https://doi.org/10.3892/ijo.15.4.669
MLA
Utsunomiya, I., Iemura, A., Yano, H., Akiba, J., Kojiro, M."Establishment and characterization of a new human hepatocellular carcinoma cell line, HAK-3, and its response to growth factors.". International Journal of Oncology 15.4 (1999): 669-744.
Chicago
Utsunomiya, I., Iemura, A., Yano, H., Akiba, J., Kojiro, M."Establishment and characterization of a new human hepatocellular carcinoma cell line, HAK-3, and its response to growth factors.". International Journal of Oncology 15, no. 4 (1999): 669-744. https://doi.org/10.3892/ijo.15.4.669