Constitutive expression of Heregulin induces apoptosis in an erbB-2 overexpressing breast cancer cell line SKBr-3.

  • Authors:
    • F K Guerra-Vladusic
    • G Scott
    • V Weaver
    • E A Vladusic
    • M S Tsai
    • C C Benz
    • R Lupu
  • View Affiliations

  • Published online on: November 1, 1999     https://doi.org/10.3892/ijo.15.5.883
  • Pages: 883-975
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Abstract

We have previously reported that Heregulin (HRG)/neu differentiation factor (NDF) induced growth arrest and cellular differentiation in breast cancer cells overexpressing erbB-2 receptor. To elucidate the cellular mechanisms underlying the growth inhibition by HRG, we developed an in vitro model by transfection of HRG cDNA into the erbB-2 overexpressing breast cancer cell line, SKBr-3. We showed that the enforced expression of HRG in SKBr-3 cells induces dramatic morphological changes and pronounced inhibition of anchorage-dependent and -independent growth. Most SKBr-3/HRG-transfected (SK/HRG) cells exhibited about 15-fold increase in size and persisted as multinucleated cells with extended flattened vacuole-filled cytoplasm with reduced cell attachment. The growth suppression of SK/HRG cells was accompanied by a reduction in S phase, the presence of a G2-M cell cycle delay, and an increase in DNA aneuploid components. In addition, DNA fragmentation assays showed that HRG induced apoptosis of SKBr-3 cells. In contrast, while HRG treatment of other erbB-2 overexpressing breast cancer cell lines led to growth arrest and cell detachment, it did not induce apoptotic features. Thus, this study demonstrates that while growth arrest and cell detachment are general effects of HRG towards erbB-2 overexpressing cells, the ability of HRG to induce apoptosis is a phenomenon confined to selective cells including SKBr-3 cells.

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Nov 1999
Volume 15 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Guerra-Vladusic F, Scott G, Weaver V, Vladusic E, Tsai M, Benz C and Lupu R: Constitutive expression of Heregulin induces apoptosis in an erbB-2 overexpressing breast cancer cell line SKBr-3.. Int J Oncol 15: 883-975, 1999
APA
Guerra-Vladusic, F., Scott, G., Weaver, V., Vladusic, E., Tsai, M., Benz, C., & Lupu, R. (1999). Constitutive expression of Heregulin induces apoptosis in an erbB-2 overexpressing breast cancer cell line SKBr-3.. International Journal of Oncology, 15, 883-975. https://doi.org/10.3892/ijo.15.5.883
MLA
Guerra-Vladusic, F., Scott, G., Weaver, V., Vladusic, E., Tsai, M., Benz, C., Lupu, R."Constitutive expression of Heregulin induces apoptosis in an erbB-2 overexpressing breast cancer cell line SKBr-3.". International Journal of Oncology 15.5 (1999): 883-975.
Chicago
Guerra-Vladusic, F., Scott, G., Weaver, V., Vladusic, E., Tsai, M., Benz, C., Lupu, R."Constitutive expression of Heregulin induces apoptosis in an erbB-2 overexpressing breast cancer cell line SKBr-3.". International Journal of Oncology 15, no. 5 (1999): 883-975. https://doi.org/10.3892/ijo.15.5.883