Molecular profiling of sporadic colorectal tumors by microsatellite analysis.

  • Authors:
    • J Gebert
    • M Sun
    • R Ridder
    • U Hinz
    • T Lehnert
    • P Möller
    • H K Schackert
    • C Herfarth
    • M von Knebel Doeberitz
  • View Affiliations

  • Published online on: January 1, 2000     https://doi.org/10.3892/ijo.16.1.169
  • Pages: 169-248
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Abstract

To investigate the prognostic value of multiple genetic alterations, individual molecular tumor profiles were established in 79 sporadic colorectal carcinomas (41 stage II and 38 stage III). Tumors were analyzed for allelic loss (LOH) and genetic instability (MSI) using 14 microsatellites intragenic to or associated with tumor suppressor or DNA mismatch repair genes. Molecular profiling identified tumors with LOH at multiple loci without microsatellite instability (MSS), tumors with high levels of LOH and low level microsatellite marker instability (MSI-L), and tumors with high levels of MSI (MSI-H), but rare LOH. K-ras mutations occurred more frequently in MSS/MSI-L carcinomas (26%) than in MSI-H colorectal tumors (10%), the latter showing a high frequency of TGFbeta type II frameshift mutations (82%). Correlation of molecular and clinical data revealed a better prognosis for stage III tumor patients displaying 5q12 loss rather than retention of heterozygosity. Thus, molecular profiling allows the identification of new prognostic markers and might facilitate the stratification of colorectal cancer patients.

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Jan 2000
Volume 16 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Gebert J, Sun M, Ridder R, Hinz U, Lehnert T, Möller P, Schackert H, Herfarth C and von Knebel Doeberitz M: Molecular profiling of sporadic colorectal tumors by microsatellite analysis.. Int J Oncol 16: 169-248, 2000
APA
Gebert, J., Sun, M., Ridder, R., Hinz, U., Lehnert, T., Möller, P. ... von Knebel Doeberitz, M. (2000). Molecular profiling of sporadic colorectal tumors by microsatellite analysis.. International Journal of Oncology, 16, 169-248. https://doi.org/10.3892/ijo.16.1.169
MLA
Gebert, J., Sun, M., Ridder, R., Hinz, U., Lehnert, T., Möller, P., Schackert, H., Herfarth, C., von Knebel Doeberitz, M."Molecular profiling of sporadic colorectal tumors by microsatellite analysis.". International Journal of Oncology 16.1 (2000): 169-248.
Chicago
Gebert, J., Sun, M., Ridder, R., Hinz, U., Lehnert, T., Möller, P., Schackert, H., Herfarth, C., von Knebel Doeberitz, M."Molecular profiling of sporadic colorectal tumors by microsatellite analysis.". International Journal of Oncology 16, no. 1 (2000): 169-248. https://doi.org/10.3892/ijo.16.1.169