The detection of p53 gene mutation using a microdissection technique in primary intracranial germ cell tumors.

  • Authors:
    • S K Kim
    • B K Cho
    • S H Paek
    • S J Hong
    • H S Kim
    • S Y Hong
    • G Choe
    • J G Chi
    • D H Nam
    • K C Wang
  • View Affiliations

  • Published online on: January 1, 2001     https://doi.org/10.3892/ijo.18.1.111
  • Pages: 111-117
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Abstract

Using a microdissection technique, the contribution of the p53 mutation to tumorigenesis and prognosis in each histological subtype of the intracranial germ cell tumors (GCTs) was evaluated. Nineteen patients had primary intracranial GCTs, including 4 germinomas (GEs), 4 teratomas (TEs), 1 mixed tumor of GE and TE, and 10 mixed GCTs containing non-germinomatous malignant germ cell tumors (NG-MGCTs). After microdissection of specific subtypes, genomic DNA was screened for mutations in exons 5-8 of the p53 gene, using the dideoxyfingerprinting (ddF) followed by direct DNA sequencing. The direct sequencing revealed a total of six mutations in PCR products derived from the five cases (26%) which showed mobility shifts in ddF. Among the six mutations detected, four were missense mutations and two were silent. Missense mutations of the p53 gene tended to occur more frequently in the NG-MGCT component than in the GE or TE components (3/15 vs. 1/12 vs. 0/13). The incidence of missense mutations was not different between the survivors (3/13) and the deceased (1/6). This study suggests the possible role of the p53 gene in the tumori-genesis of NG-MGCT. However, p53 gene mutation did not correlate with the prognosis of NG-MGCT.

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January 2001
Volume 18 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kim S, Cho B, Paek S, Hong S, Kim H, Hong S, Choe G, Chi J, Nam D, Wang K, Wang K, et al: The detection of p53 gene mutation using a microdissection technique in primary intracranial germ cell tumors.. Int J Oncol 18: 111-117, 2001
APA
Kim, S., Cho, B., Paek, S., Hong, S., Kim, H., Hong, S. ... Wang, K. (2001). The detection of p53 gene mutation using a microdissection technique in primary intracranial germ cell tumors.. International Journal of Oncology, 18, 111-117. https://doi.org/10.3892/ijo.18.1.111
MLA
Kim, S., Cho, B., Paek, S., Hong, S., Kim, H., Hong, S., Choe, G., Chi, J., Nam, D., Wang, K."The detection of p53 gene mutation using a microdissection technique in primary intracranial germ cell tumors.". International Journal of Oncology 18.1 (2001): 111-117.
Chicago
Kim, S., Cho, B., Paek, S., Hong, S., Kim, H., Hong, S., Choe, G., Chi, J., Nam, D., Wang, K."The detection of p53 gene mutation using a microdissection technique in primary intracranial germ cell tumors.". International Journal of Oncology 18, no. 1 (2001): 111-117. https://doi.org/10.3892/ijo.18.1.111