Overexpression of tissue factor pathway inhibitor-2 (TFPI-2), decreases the invasiveness of prostate cancer cells in vitro.

  • Authors:
    • S D Konduri
    • A Tasiou
    • N Chandrasekar
    • J S Rao
  • View Affiliations

  • Published online on: January 1, 2001     https://doi.org/10.3892/ijo.18.1.127
  • Pages: 127-158
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Human tissue factor pathway inhibitor-2 (TFPI-2) is a 32-kDa serine protease inhibitor that inhibits plasmin, trypsin, chymotrypsin, cathepsin G, and plasma kallikrein but not urokinase and tissue-type plasminogen activators or thrombin. After discovering that TFPI-2 expression is down-regulated or lost during tumor progression, we investigated the role of TFPI-2 in the invasiveness of the prostate cancer cell line (LNCaP). We stably transfected LNCaP cells with a 0.7-kb vector expressing TFPI-2 in the sense orientation and measured the expression of TFPI-2 protein and mRNA by these cells by western and northern blotting. Neither TFPI-2 protein nor mRNA was expressed by parental LNCaP cells or vector-transfected controls, but levels of both protein and mRNA were significantly increased in the sense-TFPI-2 clones. The sense clones were less invasive than the control cells in Matrigel invasion and spheroid migration assays. This is the first demonstration that upregulation of TFPI-2 plays a significant role in the invasive behavior of human prostate cancer cells.

Related Articles

Journal Cover

January 2001
Volume 18 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Konduri S, Tasiou A, Chandrasekar N and Rao J: Overexpression of tissue factor pathway inhibitor-2 (TFPI-2), decreases the invasiveness of prostate cancer cells in vitro.. Int J Oncol 18: 127-158, 2001
APA
Konduri, S., Tasiou, A., Chandrasekar, N., & Rao, J. (2001). Overexpression of tissue factor pathway inhibitor-2 (TFPI-2), decreases the invasiveness of prostate cancer cells in vitro.. International Journal of Oncology, 18, 127-158. https://doi.org/10.3892/ijo.18.1.127
MLA
Konduri, S., Tasiou, A., Chandrasekar, N., Rao, J."Overexpression of tissue factor pathway inhibitor-2 (TFPI-2), decreases the invasiveness of prostate cancer cells in vitro.". International Journal of Oncology 18.1 (2001): 127-158.
Chicago
Konduri, S., Tasiou, A., Chandrasekar, N., Rao, J."Overexpression of tissue factor pathway inhibitor-2 (TFPI-2), decreases the invasiveness of prostate cancer cells in vitro.". International Journal of Oncology 18, no. 1 (2001): 127-158. https://doi.org/10.3892/ijo.18.1.127