Comparative genomic hybridization and cytogenetic analysis in a bronchial adenoma: Three clones with different chromosomal aberrations

  • Authors:
    • R. Pfragner
    • R. Ullmann
    • A. Behmel
    • H. H. Popper
  • View Affiliations

  • Published online on: May 1, 2001     https://doi.org/10.3892/ijo.18.5.923
  • Pages: 923-928
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Abstract

Classical chromosomal analysis and comparative genomic hybridization (CGH) were performed in a tubular bronchial gland adenoma. Trisomy of chromosomes 2, 11, 18 and 20 and clonal loss of Y were found in cultured cells derived from two different kryotubes; this was also confirmed by CGH from one of these tubes. Cells from two other tubes, investigated by CGH only, showed gains and losses of parts of chromosome 11q in one, and in the second additional gain of the distal portion of 9q and 17q, respectively. CGH analysis of tumor DNA extracted from paraffin-embedded sections showed no chromosomal imbalances. In cell culture growth the advantage of specific clones probably altered the clone distribution. This study highlights the risk of cytogenetic analysis based on cell cultures only.

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May 2001
Volume 18 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Pfragner R, Ullmann R, Behmel A and Popper HH: Comparative genomic hybridization and cytogenetic analysis in a bronchial adenoma: Three clones with different chromosomal aberrations. Int J Oncol 18: 923-928, 2001
APA
Pfragner, R., Ullmann, R., Behmel, A., & Popper, H.H. (2001). Comparative genomic hybridization and cytogenetic analysis in a bronchial adenoma: Three clones with different chromosomal aberrations. International Journal of Oncology, 18, 923-928. https://doi.org/10.3892/ijo.18.5.923
MLA
Pfragner, R., Ullmann, R., Behmel, A., Popper, H. H."Comparative genomic hybridization and cytogenetic analysis in a bronchial adenoma: Three clones with different chromosomal aberrations". International Journal of Oncology 18.5 (2001): 923-928.
Chicago
Pfragner, R., Ullmann, R., Behmel, A., Popper, H. H."Comparative genomic hybridization and cytogenetic analysis in a bronchial adenoma: Three clones with different chromosomal aberrations". International Journal of Oncology 18, no. 5 (2001): 923-928. https://doi.org/10.3892/ijo.18.5.923