Synergistic effects of dexamethasone and genistein on the expression of Cdk inhibitor p21WAF1/CIP1 in human hepatocellular and colorectal carcinoma cells

  • Authors:
    • J.-H. Park
    • E.-J. Oh
    • Y. H. Choi
    • C.-D. Kang
    • H. S. Kang
    • D.-K. Kim
    • K. I. Kang
    • M.-A. Yoo
  • View Affiliations

  • Published online on: May 1, 2001     https://doi.org/10.3892/ijo.18.5.997
  • Pages: 997-1002
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Abstract

Previous studies have shown that dexamethasone, a synthetic glucocorticoid, can induce a G1 arrest, however, genistein, a natural isoflavonoid phytoestrogen, induces a G2/M arrest in the cell cycle progression in various cancer cell lines. A block of cell cycle checkpoint by dexamethasone and genistein correlates with a selective induction of cyclin-dependent kinase (Cdk) inhibitor p21WAF1/CIP1 in a tumor suppressor p53-independent manner and abolishment of Cdk2 phosphorylation. In the present study, the effects of dexamethasone and genistein (both singly and combined) on the expression of p21 in human hepatocellular Hep G2 and colorectal Colo320 HSR carcinoma cells were evaluated. Whereas dexamethasone mildly induced the level of p21 protein, genistein strongly increased the expression of p21 protein in our experimental condition. Both compounds also activated p21 promoter reporter constructs. The combined effects of dexamethasone and genistein on the induction of p21 protein and activation of p21 promoter were synergistic in both cell lines. These findings indicate that dexamethasone and genistein act in a synergistic fashion and have potential for combination chemotherapy for the treatment of liver and colon cancer.

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May 2001
Volume 18 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Park J, Oh E, Choi YH, Kang C, Kang HS, Kim D, Kang KI and Yoo M: Synergistic effects of dexamethasone and genistein on the expression of Cdk inhibitor p21WAF1/CIP1 in human hepatocellular and colorectal carcinoma cells. Int J Oncol 18: 997-1002, 2001
APA
Park, J., Oh, E., Choi, Y.H., Kang, C., Kang, H.S., Kim, D. ... Yoo, M. (2001). Synergistic effects of dexamethasone and genistein on the expression of Cdk inhibitor p21WAF1/CIP1 in human hepatocellular and colorectal carcinoma cells. International Journal of Oncology, 18, 997-1002. https://doi.org/10.3892/ijo.18.5.997
MLA
Park, J., Oh, E., Choi, Y. H., Kang, C., Kang, H. S., Kim, D., Kang, K. I., Yoo, M."Synergistic effects of dexamethasone and genistein on the expression of Cdk inhibitor p21WAF1/CIP1 in human hepatocellular and colorectal carcinoma cells". International Journal of Oncology 18.5 (2001): 997-1002.
Chicago
Park, J., Oh, E., Choi, Y. H., Kang, C., Kang, H. S., Kim, D., Kang, K. I., Yoo, M."Synergistic effects of dexamethasone and genistein on the expression of Cdk inhibitor p21WAF1/CIP1 in human hepatocellular and colorectal carcinoma cells". International Journal of Oncology 18, no. 5 (2001): 997-1002. https://doi.org/10.3892/ijo.18.5.997