MODULATION OF Y-79 RETINOBLASTOMA CELL-DIFFERENTIATION AND IRBP EXPRESSION BY DIBUTYRYL-CYCLIC-AMP AND LAMININ

  • Authors:
    • G FASSINA
    • G PAGLIALUNGA
    • DM NOONAN
    • GJ CHADER
    • A ALBINI
  • View Affiliations

  • Published online on: May 1, 1993     https://doi.org/10.3892/ijo.2.5.745
  • Pages: 745-751
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Abstract

Y-79 retinoblastoma cells grown on a poly-D-lysine-coated substratum can assume a photoreceptor-like morphology and they express the retina-specific interphotoreceptor-retinoid-binding protein (IRBP) mRNA in abundance. In contrast, cells in suspension culture remain morphologically undifferentiated and exhibit a much lower IRBP message level. After treatment with the differentiating agent dibutyryl cyclic AMP (dbcAMP), monolayer cultures of Y-79 cells grown in serum-containing medium show a partial morphological and biochemical differentiation towards a Muller-glial-like phenotype. This is accompanied by a decrease in IRBP mRNA level with respect to that in Y-79 cells grown on poly-D-lysine. Attached cells which had been pretreated with laminin for three days do not demonstrate an altered IRBP mRNA level in response to dbcAMP as compared to cells not pretreated. Surprisingly, however, cells in suspension culture that were exposed to laminin and then to dbcAMP showed a 3-fold increase in IRBP mRNA level. Thus, expression of an important photoreceptor-specific marker is clearly modulated by both dbcAMP and laminin. Moreover, attachment itself may be a significant factor in determination of a neural or neuro-retinal cell phenotype.

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May 1993
Volume 2 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
FASSINA G, PAGLIALUNGA G, NOONAN D, CHADER G and ALBINI A: MODULATION OF Y-79 RETINOBLASTOMA CELL-DIFFERENTIATION AND IRBP EXPRESSION BY DIBUTYRYL-CYCLIC-AMP AND LAMININ. Int J Oncol 2: 745-751, 1993
APA
FASSINA, G., PAGLIALUNGA, G., NOONAN, D., CHADER, G., & ALBINI, A. (1993). MODULATION OF Y-79 RETINOBLASTOMA CELL-DIFFERENTIATION AND IRBP EXPRESSION BY DIBUTYRYL-CYCLIC-AMP AND LAMININ. International Journal of Oncology, 2, 745-751. https://doi.org/10.3892/ijo.2.5.745
MLA
FASSINA, G., PAGLIALUNGA, G., NOONAN, D., CHADER, G., ALBINI, A."MODULATION OF Y-79 RETINOBLASTOMA CELL-DIFFERENTIATION AND IRBP EXPRESSION BY DIBUTYRYL-CYCLIC-AMP AND LAMININ". International Journal of Oncology 2.5 (1993): 745-751.
Chicago
FASSINA, G., PAGLIALUNGA, G., NOONAN, D., CHADER, G., ALBINI, A."MODULATION OF Y-79 RETINOBLASTOMA CELL-DIFFERENTIATION AND IRBP EXPRESSION BY DIBUTYRYL-CYCLIC-AMP AND LAMININ". International Journal of Oncology 2, no. 5 (1993): 745-751. https://doi.org/10.3892/ijo.2.5.745