CHARACTERIZATION OF OLIGOSACCHARIDES ON METHYLCHOLANTHRENE-INDUCED MURINE ONCOFETAL ANTIGEN USING BIOTINYLATED LECTINS

  • Authors:
    • LD GILLIS
    • AL BARSOUM
    • SF GOTTLIEB
    • JH COGGIN
  • View Affiliations

  • Published online on: May 1, 1993     https://doi.org/10.3892/ijo.2.5.817
  • Pages: 817-821
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Abstract

Biotinylated lectins were used to characterize oligosaccharide chains of the minute amounts of the 44 kD membrane-bound oncofetal antigen (OFA) derived from murine MCA 1315 tumor cells. Lectins of known sugar specificity were used to detect oligosaccharides on glycoprotein which had been previously separated electrophoretically on sodium dodecyl sulfate polyacrylamide gels (SDS-PAGE) and blotted onto nitrocellulose (NC). Oligosaccharide reactivity with biotinlylated lectins was also analyzed after glycosidase treatment of the NC blotted OFA. Results indicate MCA 1315 derived OFA possesses N-glycosylated oligosaccharide chains which are high in mannose. This result is in agreement with the high mannose glycosylation of OFA derived from 13-day mouse fetuses.

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May 1993
Volume 2 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
GILLIS L, BARSOUM A, GOTTLIEB S and COGGIN J: CHARACTERIZATION OF OLIGOSACCHARIDES ON METHYLCHOLANTHRENE-INDUCED MURINE ONCOFETAL ANTIGEN USING BIOTINYLATED LECTINS. Int J Oncol 2: 817-821, 1993
APA
GILLIS, L., BARSOUM, A., GOTTLIEB, S., & COGGIN, J. (1993). CHARACTERIZATION OF OLIGOSACCHARIDES ON METHYLCHOLANTHRENE-INDUCED MURINE ONCOFETAL ANTIGEN USING BIOTINYLATED LECTINS. International Journal of Oncology, 2, 817-821. https://doi.org/10.3892/ijo.2.5.817
MLA
GILLIS, L., BARSOUM, A., GOTTLIEB, S., COGGIN, J."CHARACTERIZATION OF OLIGOSACCHARIDES ON METHYLCHOLANTHRENE-INDUCED MURINE ONCOFETAL ANTIGEN USING BIOTINYLATED LECTINS". International Journal of Oncology 2.5 (1993): 817-821.
Chicago
GILLIS, L., BARSOUM, A., GOTTLIEB, S., COGGIN, J."CHARACTERIZATION OF OLIGOSACCHARIDES ON METHYLCHOLANTHRENE-INDUCED MURINE ONCOFETAL ANTIGEN USING BIOTINYLATED LECTINS". International Journal of Oncology 2, no. 5 (1993): 817-821. https://doi.org/10.3892/ijo.2.5.817