Expression analysis of pediatric solid tumor cell lines using oligonucleotide microarrays

  • Authors:
    • Daniel H. Wai
    • Karl-Ludwig Schaefer
    • Alexander Schramm
    • Eberhard Korsching
    • Frans Van Valen
    • Toshifumi Ozaki
    • Werner Boecker
    • Lothar Schweigerer
    • Barbara Dockhorn-Dworniczak
    • Christopher Poremba
  • View Affiliations

  • Published online on: March 1, 2002     https://doi.org/10.3892/ijo.20.3.441
  • Pages: 441-451
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Abstract

We identified patterns of differentially-expressed genes in cell lines derived from several pediatric solid tumors. Affymetrix Human Cancer G110 Arrays, carrying 1,700 cancer-associated genes, were applied to a panel of 11 cell lines originating from Ewing tumors (ETs), neuroblastomas, and malignant melanoma of soft parts. Hierarchical clustering clearly differentiated these 3 entities and revealed groups of 75, 102, and 36 gene probe-sets exhibiting tumor-type specific up-regulation in these cell lines, respectively. Whereas ET lines demonstrated increased expression of microtubule-associated protein tau (MAPT), protein phosphatase 1 regulatory subunit 1A (PPP1R1A), NIMA (never in mitosis gene a)-related kinase 2 (NEK2), and cyclin D1 (CCND1), neuroblastoma samples exhibited high expression of wingless-type mouse mammary tumor virus integration site family member 11 (WNT11), Drosophila frizzled homolog 2 (FZD2), and adenomatous polyposis coli (APC) which are involved in regulating free β-catenin levels. These genes likely maintain tumor-specific characteristics and participate in key downstream regulatory mechanisms. We also correlated the expression levels of up-regulated genes in ETs with their chromosomal localization and compared these data to the comparative genomic hybridization profiles of the cell lines. We demonstrate that gains of genetic material contribute essentially to differential gene expression.

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March 2002
Volume 20 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Wai DH, Schaefer K, Schramm A, Korsching E, Van Valen F, Ozaki T, Boecker W, Schweigerer L, Dockhorn-Dworniczak B, Poremba C, Poremba C, et al: Expression analysis of pediatric solid tumor cell lines using oligonucleotide microarrays. Int J Oncol 20: 441-451, 2002
APA
Wai, D.H., Schaefer, K., Schramm, A., Korsching, E., Van Valen, F., Ozaki, T. ... Poremba, C. (2002). Expression analysis of pediatric solid tumor cell lines using oligonucleotide microarrays. International Journal of Oncology, 20, 441-451. https://doi.org/10.3892/ijo.20.3.441
MLA
Wai, D. H., Schaefer, K., Schramm, A., Korsching, E., Van Valen, F., Ozaki, T., Boecker, W., Schweigerer, L., Dockhorn-Dworniczak, B., Poremba, C."Expression analysis of pediatric solid tumor cell lines using oligonucleotide microarrays". International Journal of Oncology 20.3 (2002): 441-451.
Chicago
Wai, D. H., Schaefer, K., Schramm, A., Korsching, E., Van Valen, F., Ozaki, T., Boecker, W., Schweigerer, L., Dockhorn-Dworniczak, B., Poremba, C."Expression analysis of pediatric solid tumor cell lines using oligonucleotide microarrays". International Journal of Oncology 20, no. 3 (2002): 441-451. https://doi.org/10.3892/ijo.20.3.441