Molecular cloning and characterization of bovine bgl-1, a novel family member of WD-40 repeat-containing lethal giant larvae tumor suppressor genes

  • Authors:
    • K.-H. Baek
    • Y.-S. Kim
    • S. Jung
    • K. Y. Lee
    • H.-K. Choi
    • K.-S. Kim
  • View Affiliations

  • Published online on: April 1, 2002     https://doi.org/10.3892/ijo.20.4.739
  • Pages: 739-744
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Abstract

In this study, we have isolated a bovine homologue bgl-1 of lethal giant larvae (lgl) tumor suppressor oncogene from bovine brain by RT-PCR using primers designed based on the conserved sequences for lgl family members. The sequence analysis showed that the bgl-1 encodes a 1,036 amino acid polypeptide with the molecular weight of approximately 112 kDa containing a domain characteristic of WD-40 proteins. The amino acid sequence of bgl-1 showed a homology of 98.3 and 87.3% identity to that of mouse and human, respectively. Northern blot analysis showed that bgl-1 was highly expressed in brain, ovary and testis, with moderate expression in liver, uterus, lung and kidney. This suggests that the bgl-1 may play essential roles in each of these organs. The complementation analysis revealed that the bovine bgl-1 partially restored the Na+ tolerance in the absence of yeast lgl homologue, suggesting that bgl-1 is a bovine homologue of the lgl family.

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April 2002
Volume 20 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Baek K, Kim Y, Jung S, Lee KY, Choi H and Kim K: Molecular cloning and characterization of bovine bgl-1, a novel family member of WD-40 repeat-containing lethal giant larvae tumor suppressor genes. Int J Oncol 20: 739-744, 2002
APA
Baek, K., Kim, Y., Jung, S., Lee, K.Y., Choi, H., & Kim, K. (2002). Molecular cloning and characterization of bovine bgl-1, a novel family member of WD-40 repeat-containing lethal giant larvae tumor suppressor genes. International Journal of Oncology, 20, 739-744. https://doi.org/10.3892/ijo.20.4.739
MLA
Baek, K., Kim, Y., Jung, S., Lee, K. Y., Choi, H., Kim, K."Molecular cloning and characterization of bovine bgl-1, a novel family member of WD-40 repeat-containing lethal giant larvae tumor suppressor genes". International Journal of Oncology 20.4 (2002): 739-744.
Chicago
Baek, K., Kim, Y., Jung, S., Lee, K. Y., Choi, H., Kim, K."Molecular cloning and characterization of bovine bgl-1, a novel family member of WD-40 repeat-containing lethal giant larvae tumor suppressor genes". International Journal of Oncology 20, no. 4 (2002): 739-744. https://doi.org/10.3892/ijo.20.4.739