Deficient R-smad/smad4 complex formation in fibroblasts growth-stimulated by TGF-β1

  • Authors:
    • David A. Lawrence
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  • Published online on: April 1, 2002     https://doi.org/10.3892/ijo.20.4.803
  • Pages: 803-806
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Abstract

Although most cell types are inhibited by TGF-β1 some mesenchymal cells are growth-stimulated by TGF-β1. Here, using two cell models, in which the normal untransformed cells (WI38 human embryo and NIH3T3 mouse fibroblasts) are stimulated to proliferate by TGF-β1 and their SV40 Large T-transformed counterparts are inhibited, we show that the two former cells are deficient in smad2-smad4 and smad3-smad4 complex formation, whereas these complexes are present in the two latter cells. In addition, endogenous smad4 levels in total cell extracts are lower in WI38 and NIH3T3 fibroblasts than in their Large T-expressing counterparts. In WI38 fibroblasts smad4 is, at best, inefficiently translocated to the nucleus. TGF-β1-mediated growth-stimulation of these normal fibroblasts does not appear to involve the presently known smads, but this situation is reversed in the Large T-transformed cells inhibited by TGF-β1.

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April 2002
Volume 20 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Lawrence DA: Deficient R-smad/smad4 complex formation in fibroblasts growth-stimulated by TGF-β1. Int J Oncol 20: 803-806, 2002
APA
Lawrence, D.A. (2002). Deficient R-smad/smad4 complex formation in fibroblasts growth-stimulated by TGF-β1. International Journal of Oncology, 20, 803-806. https://doi.org/10.3892/ijo.20.4.803
MLA
Lawrence, D. A."Deficient R-smad/smad4 complex formation in fibroblasts growth-stimulated by TGF-β1". International Journal of Oncology 20.4 (2002): 803-806.
Chicago
Lawrence, D. A."Deficient R-smad/smad4 complex formation in fibroblasts growth-stimulated by TGF-β1". International Journal of Oncology 20, no. 4 (2002): 803-806. https://doi.org/10.3892/ijo.20.4.803