Molecular cloning and characterization of WRCH2 on human chromosome 15q15

  • Authors:
    • Masaru Katoh
  • View Affiliations

  • Published online on: May 1, 2002     https://doi.org/10.3892/ijo.20.5.977
  • Pages: 977-982
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Abstract

WNT signals are transduced to the JNK pathway, the Ca2+-releasing pathway, or the β-catenin - TCF pathway through seven-transmembrane-type WNT receptors encoded by Frizzled genes (FZD1-FZD10). WRCH1/ARHV and CDC42 are potentially implicated in the WNT-JNK pathway. Here, WRCH2/ARHV cDNAs were isolated by using bioinformatics and cDNA-PCR. WRCH2 gene, consisting of at least 3 exons, encoded a 236-amino-acid protein with proline-rich domain and GTPase domain. WRCH2 was homologous to WRCH1 (55.4% total-amino-acid identity) and CDC42 (43.5% total-amino-acid identity). WRCH2 gene was located on human chromosome 15q15, which is one of fragile sites in the human genome. A single nucleotide substitution (632 G↷A) was identified between WRCH2 cDNA and human genome draft sequences, which resulted in Arg177Lys amino-acid substitution. WRCH2 mRNA was relatively highly expressed in pancreas, placenta, and fetal brain. WRCH2 mRNA was over-expressed in TMK1 (gastric cancer), Hs700T (pancreatic cancer), HeLa S3 (cervical cancer), and A549 (lung cancer). WRCH2 mRNA was moderately expressed in MKN74, MKN45, MKN28, KATO-III (gastric cancer), HL-60 (pro-myelocytic leukemia), Raji (Burkitt's lymphoma), and SW480 (colorectal cancer). WRCH2 mRNA was up-regulated in 3 out of 8 cases of primary gastric cancer. Because Wrch1 can activate PAK1 and JNK1, and induce filopodium formation and stress fiber dissolution, over-expression of WRCH2 mRNA in human cancer cells might also lead to more malignant phenotype.

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May 2002
Volume 20 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Katoh M: Molecular cloning and characterization of WRCH2 on human chromosome 15q15. Int J Oncol 20: 977-982, 2002
APA
Katoh, M. (2002). Molecular cloning and characterization of WRCH2 on human chromosome 15q15. International Journal of Oncology, 20, 977-982. https://doi.org/10.3892/ijo.20.5.977
MLA
Katoh, M."Molecular cloning and characterization of WRCH2 on human chromosome 15q15". International Journal of Oncology 20.5 (2002): 977-982.
Chicago
Katoh, M."Molecular cloning and characterization of WRCH2 on human chromosome 15q15". International Journal of Oncology 20, no. 5 (2002): 977-982. https://doi.org/10.3892/ijo.20.5.977