Expression of the potential novel gene E6DG1 downregulated by the E6 protein of human papillomavirus type 16 is correlated with anchorage-independent growth

  • Authors:
    • Seiichiro Sakao
    • Tomohiro Hashimoto
    • Yuji Shino
    • Takeshi Saito
    • Satoshi Kado
    • Hiroshi Sashiyama
    • Koichiro Tatsumi
    • Takayuki Kuriyama
    • Hiroshi Shirasawa
  • View Affiliations

  • Published online on: August 1, 2002     https://doi.org/10.3892/ijo.21.2.273
  • Pages: 273-279
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Abstract

A novel gene transcript that is downregulated by HPV16 E6 protein was identified in mouse cells using differential hybridization and designated E6DG1. The cloned cDNA of E6DG1 was 1.3 kb in length and contained a small ORF potentially encoding a polypeptide of 45 amino acids. In vitro transcription and translation of E6DG1 cDNA resulted in a product of approximately 7 kDa and Western blot analysis using antibodies for E6DG1 peptide detected 7 and 14 kDa proteins. Downregulation of E6DG1 mRNA levels in cells expressing HPV16 E6 protein was observed at subconfluent cell densities, but not in confluent cells. Repression of E6DG1 protein enhanced the anchorage-independent growth and weakened the cell adhesion in Panc1 cells. Immunofluorescence analysis revealed the localization of E6DG1 within the nucleus. These results indicate that the E6DG1 protein may function in a signaling pathway related to anchorage-independent growth and adhesion control.

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August 2002
Volume 21 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Sakao S, Hashimoto T, Shino Y, Saito T, Kado S, Sashiyama H, Tatsumi K, Kuriyama T and Shirasawa H: Expression of the potential novel gene E6DG1 downregulated by the E6 protein of human papillomavirus type 16 is correlated with anchorage-independent growth. Int J Oncol 21: 273-279, 2002
APA
Sakao, S., Hashimoto, T., Shino, Y., Saito, T., Kado, S., Sashiyama, H. ... Shirasawa, H. (2002). Expression of the potential novel gene E6DG1 downregulated by the E6 protein of human papillomavirus type 16 is correlated with anchorage-independent growth. International Journal of Oncology, 21, 273-279. https://doi.org/10.3892/ijo.21.2.273
MLA
Sakao, S., Hashimoto, T., Shino, Y., Saito, T., Kado, S., Sashiyama, H., Tatsumi, K., Kuriyama, T., Shirasawa, H."Expression of the potential novel gene E6DG1 downregulated by the E6 protein of human papillomavirus type 16 is correlated with anchorage-independent growth". International Journal of Oncology 21.2 (2002): 273-279.
Chicago
Sakao, S., Hashimoto, T., Shino, Y., Saito, T., Kado, S., Sashiyama, H., Tatsumi, K., Kuriyama, T., Shirasawa, H."Expression of the potential novel gene E6DG1 downregulated by the E6 protein of human papillomavirus type 16 is correlated with anchorage-independent growth". International Journal of Oncology 21, no. 2 (2002): 273-279. https://doi.org/10.3892/ijo.21.2.273