CYFRA 21-1 is released in TNF-α-induced apoptosis in the hepatocellular carcinoma cell line HuH-7

  • Authors:
    • Fei Wu
    • Jiro Fujita
    • Masayuki Murota
    • Jia-Qing Li
    • Toshihiko Ishida
    • Mikio Nishioka
    • Yuji Imaida
    • Shigeki Kuriyama
  • View Affiliations

  • Published online on: August 1, 2002     https://doi.org/10.3892/ijo.21.2.441
  • Pages: 441-445
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Many types of cancer cells widely express cytokeratin 19 (CK19). Clinical investigations have suggested that serum CYFRA 21-1, a fragment of CK19, is one of the most useful tumor markers. In the present study, we hypothesized that released CYFRA 21-1 is closely associated with cellular apoptosis during tumor growth. Apoptosis was induced by tumor necrosis factor-α (TNF-α) in the HuH-7 hepatocellular carcinoma cell line. Both TNF-α-treated and non-treated cells of HuH-7 were simultaneously examined by immunoradiometric assay, annexin-V apoptosis analysis, immunohistochemical staining and colorimetric protease measurement. Levels of CYFRA 21-1 increased significantly in TNF-α-treated cells displaying a high percentage of apoptosis, granular-like aggregation of CK19, and elevated activity of caspase-3 in contrast to non-treated cells. Levels of CYFRA 21-1 decreased significantly after caspase-3 was inhibited in TNF-α-treated cells. Thus, the release of CYFRA 21-1 may suggest cellular apoptosis in the process of tumor growth.

Related Articles

Journal Cover

August 2002
Volume 21 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wu F, Fujita J, Murota M, Li J, Ishida T, Nishioka M, Imaida Y and Kuriyama S: CYFRA 21-1 is released in TNF-α-induced apoptosis in the hepatocellular carcinoma cell line HuH-7. Int J Oncol 21: 441-445, 2002
APA
Wu, F., Fujita, J., Murota, M., Li, J., Ishida, T., Nishioka, M. ... Kuriyama, S. (2002). CYFRA 21-1 is released in TNF-α-induced apoptosis in the hepatocellular carcinoma cell line HuH-7. International Journal of Oncology, 21, 441-445. https://doi.org/10.3892/ijo.21.2.441
MLA
Wu, F., Fujita, J., Murota, M., Li, J., Ishida, T., Nishioka, M., Imaida, Y., Kuriyama, S."CYFRA 21-1 is released in TNF-α-induced apoptosis in the hepatocellular carcinoma cell line HuH-7". International Journal of Oncology 21.2 (2002): 441-445.
Chicago
Wu, F., Fujita, J., Murota, M., Li, J., Ishida, T., Nishioka, M., Imaida, Y., Kuriyama, S."CYFRA 21-1 is released in TNF-α-induced apoptosis in the hepatocellular carcinoma cell line HuH-7". International Journal of Oncology 21, no. 2 (2002): 441-445. https://doi.org/10.3892/ijo.21.2.441