Hyperthermia improves cellular immune response to human hepatocellular carcinoma subsequent to co-culture with tumor lysate pulsed dendritic cells

  • Authors:
    • Gerd Schueller
    • Anton Stift
    • Josef Friedl
    • Peter Dubsky
    • Thomas Bachleitner-Hofmann
    • Thomas Benkoe
    • Raimund Jakesz
    • Michael Gnant
  • View Affiliations

  • Published online on: June 1, 2003     https://doi.org/10.3892/ijo.22.6.1397
  • Pages: 1397-1402
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Dendritic cells play a major role in cellular immunity. The crucial steps of antigen presentation and processing by DCs may be limiting factors for adoptive cellular immunotherapy. Here, we investigated whether hyperthermia of human hepatocellular carcinoma (HCC) cells induces enhanced cytotoxic cellular immune response. Peripheral blood mononuclear cell (PBMC)-derived DCs were pulsed with tumor cell lysate of the human HCC cell line HepG2, which had been heat shocked prior to incubation for 5 h. Subsequent to TNFα-induced maturation DCs were co-cultured with autologous CD4+ and/or CD8+ cells, and T cell mediated cytolysis of HepG2 cells was assessed. We observed enhanced CD4+/8+ cellular cytotoxicity against HepG2 cells subsequent to co-culture with the heat shocked tumor lysate pulsed DCs as compared to pulsing DCs with lysate of non-heat shocked tumor cells. The improved cellular immune response can be related to enhanced expression of HSP 70 and 90 in HepG2 cells upon hyperthermia.

Related Articles

Journal Cover

June 2003
Volume 22 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Schueller G, Stift A, Friedl J, Dubsky P, Bachleitner-Hofmann T, Benkoe T, Jakesz R and Gnant M: Hyperthermia improves cellular immune response to human hepatocellular carcinoma subsequent to co-culture with tumor lysate pulsed dendritic cells. Int J Oncol 22: 1397-1402, 2003
APA
Schueller, G., Stift, A., Friedl, J., Dubsky, P., Bachleitner-Hofmann, T., Benkoe, T. ... Gnant, M. (2003). Hyperthermia improves cellular immune response to human hepatocellular carcinoma subsequent to co-culture with tumor lysate pulsed dendritic cells. International Journal of Oncology, 22, 1397-1402. https://doi.org/10.3892/ijo.22.6.1397
MLA
Schueller, G., Stift, A., Friedl, J., Dubsky, P., Bachleitner-Hofmann, T., Benkoe, T., Jakesz, R., Gnant, M."Hyperthermia improves cellular immune response to human hepatocellular carcinoma subsequent to co-culture with tumor lysate pulsed dendritic cells". International Journal of Oncology 22.6 (2003): 1397-1402.
Chicago
Schueller, G., Stift, A., Friedl, J., Dubsky, P., Bachleitner-Hofmann, T., Benkoe, T., Jakesz, R., Gnant, M."Hyperthermia improves cellular immune response to human hepatocellular carcinoma subsequent to co-culture with tumor lysate pulsed dendritic cells". International Journal of Oncology 22, no. 6 (2003): 1397-1402. https://doi.org/10.3892/ijo.22.6.1397