A hypoxic response induced in MatLyLu cells by cobalt chloride results in an enhanced angiogenic response by the chick chorioallantoic membrane

  • Authors:
    • T. Van Lieshout
    • J. Stanisz
    • V. Espiritu
    • M. Richardson
    • G. Singh
  • View Affiliations

  • Published online on: September 1, 2003     https://doi.org/10.3892/ijo.23.3.745
  • Pages: 745-750
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Abstract

Hypoxia is an important regulator of hypoxia inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) expression. Using Dunning rat prostate tumour cells (MatLyLu) we examined the induction of HIF-1α and VEGF by hypoxia and if the induction of these proteins would lead to angiogenesis in the chick chorioallantoic membrane (CAM). MatLyLu cells were exposed to 1% O2, in 5.25% CO2 and 94.75% N2, or treated with 100 mM CoCl2 to simulate hypoxia. Conditioned medium was analyzed using Western blots for HIF-1α or VEGF. VEGF levels were quantified using ELISA. Hypoxia significantly increased both HIF-1α and VEGF protein production. MatLyLu cells (1x106 viable cells in 50 µl of medium) grown under normoxic conditions induced in angiogenesis in the CAM, evaluated by the formation of a spoke wheel, and in cross sections by the number of blood vessels, following 5-day culture. This response was significantly increased using CoCl2-treated cells. Culture medium alone with or without CoCl2 was not angiogenic. These results provide direct evidence for the role of hypoxia in the induction of HIF-1α and VEGF which act as key angiogenic signals.

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September 2003
Volume 23 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Van Lieshout T, Stanisz J, Espiritu V, Richardson M and Singh G: A hypoxic response induced in MatLyLu cells by cobalt chloride results in an enhanced angiogenic response by the chick chorioallantoic membrane. Int J Oncol 23: 745-750, 2003
APA
Van Lieshout, T., Stanisz, J., Espiritu, V., Richardson, M., & Singh, G. (2003). A hypoxic response induced in MatLyLu cells by cobalt chloride results in an enhanced angiogenic response by the chick chorioallantoic membrane. International Journal of Oncology, 23, 745-750. https://doi.org/10.3892/ijo.23.3.745
MLA
Van Lieshout, T., Stanisz, J., Espiritu, V., Richardson, M., Singh, G."A hypoxic response induced in MatLyLu cells by cobalt chloride results in an enhanced angiogenic response by the chick chorioallantoic membrane". International Journal of Oncology 23.3 (2003): 745-750.
Chicago
Van Lieshout, T., Stanisz, J., Espiritu, V., Richardson, M., Singh, G."A hypoxic response induced in MatLyLu cells by cobalt chloride results in an enhanced angiogenic response by the chick chorioallantoic membrane". International Journal of Oncology 23, no. 3 (2003): 745-750. https://doi.org/10.3892/ijo.23.3.745